Abstract
Obejective To explore protective effect of miR-29c on cerebral ischemia-reperfusion injury in rats. Methods Forty eight SD rats were randomly divided into sham group , model group, miR-29c NC group and miR-29c inhibitor group .Except the sham operation group , the other three groups were established by using ligation of middle cerebral artery.Twenty four hours after the operation , neurological behavior score , cerebral infarction volume, cell apoptosis, the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA), cysteine aspartic acid protease 3 (caspase 3), caspase 8, caspase 9, and the expression of miR-29c, Bcl-2, Bax, Bak1, cleaved caspase 3, cleaved caspase 8 and cleaved caspase 9.Results Compared with sham group, neurological behavior score , cerebral infarction volume, cell apoptosis rate was increased , SOD th the expression of miR-29c, activity was decreased, the level of MDA, caspase 3, caspase 8 and caspase 9 activity was increased.The expression of Bcl-2 was down-regulated, the expression of Bax, Bak1, cleaved caspase 3, cleaved caspase 8 and cleaved caspase 9 expression was up-regulated in model group, miR-29c NC group.The differences were also statistically significant (P<0.01).Compared with model group, miR-29c NC group, the expression of miR-29c, neurological behavior score , cerebral infarction volume percentage , cell apoptosis rate was decreased, SOD activity was increased, the level of MDA, caspase 3, caspase 8 and caspase 9 activity was decreased.The expression of Bcl-2 was up-regulated, the expression of Bax, Bak1, cleaved caspase 3, cleaved caspase 8 and cleaved caspase 9 expression was down-regulated in miR-29c inhibitor group .The differences were also statistically significant (P<0.01).Conclusion miR-29c had a protective effect on cerebral ischemia-reperfusion injury in rats, which was related to improve the antioxidant capacity and regulation of apoptosis related protein expression .关键词
miR-29c/脑缺血再灌注/细胞凋亡Key words
miR-29c/cerebral ischemia-reperfusion/cell apoptosis分类
医药卫生
