摘要
Abstract
Objective To investigate Interleukin 6 ( IL-6 ) , superoxide dismutase ( SOD ) , malondialdehyde ( MDA) in lung tissues of a rat acute pulmonary edema model induced by acute hypoxia .Methods 50 adult Wistar rats were randomly divided into group A (normal),B ( acute pulmonary edema,hypoxia for 24 h),C (acute pulmonary edema, hypoxia for 48h),D(acute pulmonary edema,hypoxia for 72 h) and E (dexamethasone treatment,hypoxia for 72h).The model of acute pulmonary edema was established by intraperitoneal injection with 6% ammonium chloride .In group E, dexamethasone (6mg/kg) was injected in tail vein following intraperitoneal injection of 6% ammonium chloride.The rats were killed 24(group B), 48 (C) and 72 hours(D&E) later.Plasma was isolated and lungs were removed .MDA,SOD in lung tissues and IL-6 in plasma were analyzed by ELISA .Results The wet weight of lung tissues were significantly increased in group B , C and D compared to group A ( P<0.05 ) .The lung tissues of group A had no obvious congestion and edema,and the morphology of lung tissues was normal (P>0.05).Pulmonary edema,interstitial spaces and alveolar filled fluid can be seen in group B ,C,D and transparent membrane formed within alveoli .The lung tissue congestion and edema in group D were the most obvious (P<0.05).The above changes obviously improved in group E .Compared with group A,MDA,IL-6 increased and SOD decreased in lung tissues of group C and D .Compared with group D , MDA was decreased and SOD was increased in lung tissues of group E and IL-6 was also decreased significantly in plasma ( P <0.05).Conclusion The occurrence of acute pulmonary edema related to oxidative stress and decreased antioxidant capacity.Increased free radical is an important mechanism of pulmonary edema .SOD,MDA and IL-6 in the lung tissue may be indexes for the prognosis .关键词
急性肺水肿/大鼠模型/丙二醛/超氧化物歧化酶/白细胞介素-6Key words
Acute pulmonary edema/Rat model/Malondialdehyde ( MDA )/Superoxide dismulase ( SOD )/Interleukin 6(IL-6)分类
医药卫生
