摘要
Abstract
Objective To explore experimental study of Jiawei Danshen Yin on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into myocardium-like cells in vitro.Methods Rat BMSCs were isolated by adherent method , and their surface antigen were detected by flow cytometry .The maximal non-toxic concentration of Jiawei Danshen Yin was measured by MTT method .The isolated and purified BMSCs were divided into four groups:normal control group , Jiawei Danshen Yin group , 5-azacitidine group , Jiawei Danshen Yin plus 5-azacitidine group .The expression of desmin ,α-myosin heavy chain (α-MHC) and cardiac troponin T ( cTnT) protein was detected by western blot .The expression of GATA-4, gap junction connexin 43 (CX43) and Nkx2-5 mRNA was detected by RT-PCR.Results The percentage of CD90 positive cells in the rat BMSCs was higher than 95%, and the proportions of CD 34 positive and CD45 positive cells was lower than 5%.The maximum toxic concentration of Jiawei Danshen Yin on BMSCs was 0.625 g/L.Compared with the normal control group , the expression of desmin ,α-MHC and cardiac cTnT protein was up-regulated, the expression of GATA-4, CX43 and Nkx2-5 mRNA was up-regulated in Jiawei Danshen Yin group , 5-azacitidine group and Jiawei Danshen Yin plus 5-azacitidine group.The difference was statistically significant (P<0.01).Compared with Danshen Yin group or 5-azacitidine group, the expression of desmin , α-MHC and cardiac cTnT protein was up-regulated, the expression of GATA-4, CX43 and Nkx2-5 mRNA was up-regulated in Jiawei Danshen Yin plus 5-azacitidine group.The difference was statistically significant ( P <0.01 ) . Conclusion Jiawei Danshen Yin could induce BMSCs differentiation into myocardium-like cells, which had a synergistic effect with 5-azacitidine.关键词
加味丹参饮/骨髓间充质干细胞/心肌样细胞/5-氮胞苷/分化Key words
Jiawei Danshen Yin/Bone marrow mesenchymal stem cells/Myocardium-like cells/5-azacitidine/differentiation分类
医药卫生
