中国比较医学杂志2016，Vol.26Issue(11)：49-54,6.DOI:10.3969/j.issn.1671-7856.2016.11.009
siRNA沉默Bmi－1基因表达对宫颈癌细胞增殖的影响
Effect of siRNA silencing Bmi-1 gene on cell proliferation of cervical cancer cells
张冬丽 1王伟 1张红霞1
作者信息
- 1. 河南大学淮河医院妇产科，河南开封 475000
- 折叠
摘要
Abstract
Objective To explore effect of siRNA silencing B-cell specific Moloney murine leukemiavirus integration site ( Bmi-1 ) gene expression on cell proliferation of cervical cancer cells .Methods Hela cells were transferred with control and siRNA Bmi-1.The expression of Bmi-1 in Hela cell was examed by Realtime PCR and western blot.The cell viability was detected by MTT .The cell apoptosis was examed by Annexin V-PI flow cytometry and Hoechst staining.Cell cycle was detected by flow cytometry .The expression of p16, p53, CyclinD1 and Rb was detected by western blot.Results Compared with control group , the expression of Bmi-1 protein and mRNA was down-regulated, the cell viability was decreased , cell apoptotic rate was increased , Cell cycle was arrested in the G 1 phase, the expression of p16 was up-regulated, the expresson of p53 and CyclinD1 down-regulated, the phosphorylation of Rb was inhibited in siRNA Bmi-1 group.The difference was statistically significant (P<0.01).Conclusion Bmi-1 inhibited Hela cell proliferation via regulation of expression of cell cycle related protein .关键词
B细胞特异的莫洛尼白血病病毒插入位点1基因( Bmi-1)/宫颈癌Hela细胞/增殖Key words
B-cell specific Moloney murine leukemiavirus integration site ( Bmi-1)/Human cervical cancer Hela cells/Proliferation分类
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张冬丽,王伟,张红霞..siRNA沉默Bmi－1基因表达对宫颈癌细胞增殖的影响[J].中国比较医学杂志,2016,26(11):49-54,6.
