中国比较医学杂志2016，Vol.26Issue(11)：72-76,5.DOI:10.3969/j.issn.1671-7856.2016.11.013
自发性高血压大鼠心肌组织microRNA－97a与TGF－β1蛋白表达的改变及意义
Expression of microRNA-97a and TGF-β1 protein in myocardium of spontaneously hypertensive rats and its significance
摘要
Abstract
Objective To observe the changes of microRNA-97a and transforming growth factor β1 ( TGF-β1) protein in the myocardium of spontaneously hypertensive rats ( SHR ) .Methods 8 male spontaneously hypertensive (SHR)rats as SHR group, 8 male Wistar rats for control group, Arterial blood pressure was detected by a noninvasive blood pressure measurement and analysis system .8 weeks after femoral artery ,HE staining was used to observe the changes of morphology of the rat heart , the expression of miRNA-97a was checked by Real-time PCR method for detection of rat heart.Western blot was used to detect the TGF-β1, angiotensin II (Ang II) protein and type I collagen (Col I) and type III collagen ( Col III ) protein expression level .Results In SHR group , systolic blood pressure and diastolic blood pressure increased significantly , TGF -β1 protein and Col I and Col III protein expression levels increased significantly , miRNA-97a expression levels were significantly lower than that of control group (P <0.01).Real-time PCR results showed that the level of miRNA-97a expression in SHR group was (24.6 +4.7)%in control group, the expression level of miRNA-97a in SHR group was negatively correlated with the expression of TGF-β1 (r=0.785, P <0.01) in group. Conclusion The level of miRNA-97a is down-regulated along with the up-regulation of TGF-β1 protein expression and collagen synthesis in the myocardial tissues of SHR .miRNA-97a and TGF-β1 may be involved in myocardial fibrosis in SHR.关键词
Micro RNA-97a/转化生长因子β1/自发性高血压大鼠/心肌纤维化Key words
MicroRNA-97a/Transforming growth factor β1 ( TGF -β1 )/Spontaneously hypertensive rats/Myocardial fibrosis引用本文复制引用
郑婧..自发性高血压大鼠心肌组织microRNA－97a与TGF－β1蛋白表达的改变及意义[J].中国比较医学杂志,2016,26(11):72-76,5.基金项目
贵州省科技合作计划项目支助（黔科合LH字（2015）7423）。 （）
