中国药房2016，Vol.27Issue(33)：4689-4691,3.DOI:10.6039/j.issn.1001-0408.2016.33.27
蒙药大栀子超微饮片的质量标准研究
Study on the Quality Standard of Ultramicro Decoction Piece of Meng Medicine Gardenia jasminoides
摘要
Abstract
OBJECTIVE:To establish the quality standard of ultramicro decoction piece of Meng medicine Gardenia jasminoides. METHODS:Microscopic identification character of the ultramicro decoction piece was observed;TLC was adopted for its qualitative identification;moisture,ash,extract were detected;HPLC was used for the content determination of geniposide:the column was Kro-masil C18 with mobile phase of acetonitrile-water(15∶85,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 238 nm,col-umn temperature was 30℃,and injection volume was 10μl. RESULTS:Cell wall was completely broken and the particle sizes were 5-60μm. TLC spots were clear and well separated. The moisture was 3.89%-5.55%,ash was 3.13%-5.22%,acid-insoluble ash was 0.16%-0.80%,and extract was 29.2%-43.4%. The linear range of geniposide was 1.56-7.82μg/ml(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 2.0%;recovery was 100.17%-102.04%(RSD=1.07%,n=6). CONCLUSIONS:The standard can be used for the quality control of ultramicro decoction piece of Meng medicine G. jasminoides.关键词
蒙药大栀子/超微饮片/栀子苷/质量标准Key words
Gardenia jasminoides/Ultramicro decoction piece/Geniposide/Quality standard分类
医药卫生引用本文复制引用
白万富,鞠爱华,张静,蔡丽娟,庄志鹤..蒙药大栀子超微饮片的质量标准研究[J].中国药房,2016,27(33):4689-4691,3.基金项目
国家科技支撑计划课题(No.2012BAI28B01) （No.2012BAI28B01）
