中国药房2016，Vol.27Issue(33)：4692-4694,4695,4.DOI:10.6039/j.issn.1001-0408.2016.33.28
HPLC法同时测定风湿关节炎片中4种成分的含量Δ
Simultaneous Setermination of Four Components of Rheumatoid Arthritis Tablet by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of ephedrine hydrochloride,pseudoephedrine hydrochloride,brucine,strychnine in Rheumatoid arthritis tablet. METHODS:HPLC was performed on column of XTerra-C18 with mobile phase of acetonitrile-mixed solution(pH was adjusted to 2.8 with phosphate acid)composing of 0.01 mol/L sodium heptane-sulfonate solution and equal quantity of 0.01 mol/L potassium dihydrogen phosphate solution at a flow rate of 1.0 ml/min,detection wavelength was 206 nm for ephedrine hydrochloride and pseudoephedrine hydrochloride,260 nm for brucine and strychnine,col-umn temperature was 30℃,and injection volume was 10 µl. RESULTS:The linear range was 0.916-18.320 0 µg/ml for pseudo-ephedrine hydrochloride(r=0.999 6),10.034-200.680 0 µg/ml for ephedrine hydrochloride(r=0.999 7),8.693-121.700 0 µg/ml for brucine(r=0.999 9) and 9.696-193.920 0 µg/ml for strychnine(r=0.999 9);limits of quantification were 0.03,0.07,0.04,0.03 ng,limits of detection were 0.01,0.02,0.01,0.01 ng;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.30%-99.79%(RSD=0.96%,n=6),95.81%-99.36%(RSD=0.38%,n=6),95.34%-99.93%(RSD=2.00%,n=6)and 95.59%-99.80%(RSD=1.76%,n=6). CONCLUSIONS:The method is simple,accurate,and can be used for the simultaneous determination of ephedrine hydrochloride,pseudoephedrine hydrochloride,brucine,strychnine in Rheumatoid ar-thritis tablet.关键词
高效液相色谱法/风湿关节炎片/盐酸麻黄碱/盐酸伪麻黄碱/马钱子碱/士的宁/含量测定Key words
HPLC/Rheumatoid arthritis tablet/Ephedrine hydrochloride/Pseudoephedrine hydrochloride/Brucine/Strych-nine/Content determination分类
医药卫生引用本文复制引用
逯雯洁,刘亚蓉,韩晓萍,刘学良..HPLC法同时测定风湿关节炎片中4种成分的含量Δ[J].中国药房,2016,27(33):4692-4694,4695,4.基金项目
国家药品计划抽验项目 （）
