中国药房2016，Vol.27Issue(33)：4698-4700,3.DOI:10.6039/j.issn.1001-0408.2016.33.30
HPLC-DAD多波长法同时测定丁氯麻滴眼剂中3种成分的含量
Simultaneous Determination of 3 Components in Dinglyuma Eye Drop by HPLC-DAD at Multi-wavelength
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of chloramphenicol,ephedrine hydrochlo-ride and tetracaine hydrochloride in Dinglyuma eye drop. METHODS:HPLC-DAD at muliti-wavelength was performed on the col-umn of ZORBAX SB-C18 with mobile phase of pH 3.0 Phosphate buffer-acetonitrile (gradient elution),column temperature was room temperature,flow rate was 1.0 ml/min,injection volume was 20 μl,and the detection wavelength was 277 nm for chloram-phenicol, 210 nm for ephedrine hydrochloride and 310 nm for tetracaine hydrochloride. RESULTS:The linear range was 12.56-125.6 µg/ml for chloramphenicol(r=0.999 8),25.55-255.5 µg/ml(r=0.999 9) for ephedrine hydrochloride and 25.25-252.5 µg/ml for tetracaine hydrochloride(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recov-eries were 98.39-100.44%(RSD=0.79%,n=9),100.40-101.74%(RSD=0.66%,n=9)and 98.83-100.67%(RSD=0.70%,n=9). CONCLUSIONS:The method is sensitive,accurate and specific,and can be used for the quality control of Dinglyuma eye drop.关键词
高效液相色谱-二极管阵列检测器/丁氯麻滴眼剂/氯霉素/盐酸麻黄碱/盐酸丁卡因/含量测定Key words
HPLC-DAD/Dinglyuma eye drop/Chloramphenicol/Ephedrine hydrochloride/Tetracaine hydrochloride/Con-tent determination分类
医药卫生引用本文复制引用
杨平,曾蔚欣,孙路路..HPLC-DAD多波长法同时测定丁氯麻滴眼剂中3种成分的含量[J].中国药房,2016,27(33):4698-4700,3.基金项目
首都卫生发展科研专项项目（No.首发2011-2008-01）；北京市卫生和计划生育委员会科技成果和适宜技术推广项目 （）
