中国药房2016，Vol.27Issue(33)：4701-4703,4704,4.DOI:10.6039/j.issn.1001-0408.2016.33.31
石柑子药材的HPLC指纹图谱研究
Study on the HPLC Fingerprint of Pothos chinensis
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprint of Pothos chinensis. METHODS:HPLC was performed on the col-umn of Thermo Hypersil ODS-2 with mobile phase of methanol-0.4% phosphoric acid solution(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 300 nm,column temperature was 30 ℃,and injection volume was 10 μl. Using ferulic acid tyramine peak as reference,HPLC spectrum of 24 batches of medicinal herbs was determined,and TCM Chromatographic Finger-print Similarity Evaluation System(2004 edition)was adopted for common peak identification and similarity evaluation. RESULTS:There were 16 common peaks totally in the 24 batches ,similarity of the 21 batches of medicinal materials were higher than 0.8. CONCLUSIONS:The established fingerprint is specific,stable,and can provide basis for quality control of P. chinensis.关键词
石柑子/高效液相色谱法/指纹图谱Key words
Pothos chinensis/HPLC/Fingerprint分类
医药卫生引用本文复制引用
罗文汇,刘布鸣,彭丽诗,邱宏聪,陈昭,江洁怡..石柑子药材的HPLC指纹图谱研究[J].中国药房,2016,27(33):4701-4703,4704,4.基金项目
广西中药质量标准研究重点实验室开放课题项目（No.桂中重开201302）；广西科学研究与技术开发计划项目 （）
