中国药房2016，Vol.27Issue(33)：4719-4721,3.DOI:10.6039/j.issn.1001-0408.2016.33.37
HPLC法同时测定不同产地羊蹄药材中大黄素、大黄酚、大黄素甲醚的含量
Simultaneous Determination of Emodin,Chrysophanol and Physcion in Rumex japonicus from Different Pro-ducing Areas by HPLC
何盛江 1聂阳 1陈刚 2焦豪妍 1杨燕军1
作者信息
- 1. 广东省中药研究所，广州 510520
- 2. 解放军第113医院，浙江宁波 315040
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for contents determination of emodin,chrysophanol and physcion in Rumex ja-ponicus,and compared the differences of components in medicinal materials from different producing areas. METHODS:HPLC was performed on the column of Agilent C18 with mobile phase of methanal-0.1% phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 35 ℃,and injection volume was 20 μl. RE-SULTS:The linear range was 2.04-24.48 μg/ml for emodin (r=0.999 7),2.00-24.00 μg/ml for chrysophanol (r=0.999 5) and 1.02-12.24 μg/ml for physcion(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recover-ies were 97.09%-102.65%(RSD=1.79%,n=9),97.10%-100.31%(RSD=0.97%,n=9)and 95.45%-100.18%(RSD=1.45%, n=9). CONCLUSIONS:The method is simple with high precision,stability and reproducibility,and can be used for the simulta-neous determination of emodin,chrysophanol and physcion in R. japonicas.There are obvious differences in medicinal materials from different producing areas.关键词
高效液相色谱法/羊蹄/大黄素/大黄酚/大黄素甲醚/含量测定Key words
HPLC/Rumex japonicus/Emodin/Chrysophanol/Physcion/Content determination分类
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何盛江,聂阳,陈刚,焦豪妍,杨燕军..HPLC法同时测定不同产地羊蹄药材中大黄素、大黄酚、大黄素甲醚的含量[J].中国药房,2016,27(33):4719-4721,3.
