Abstract
OBJECTIVE:To establish a method for the simultaneous determination of vitamin B6,hydrochlorothiazide,rutin, promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet. METHODS:HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.2% phosphoric acid (gradient elution) at a flow rate of 1.5 ml/min, detection wavelength was 291 nm (0-3 min,vitamin B6),273 nm(3-5 min,hydrochlorothiazide),251 nm(5-11 min,rutin,pro-methazine hydrochloride)and 218 nm(11 min,reserpine),column temperature was 30 ℃,and injection volume was 20 μl. RE-SULTS:The linear range was 84.0-1 344.0 μg/ml for vitamin B6(r=0.999 8),171.0-2 736.0 μg/ml for hydrochlorothiazide(r=0.999 2),428.0-6 848.0 μg/ml for rutin(r=0.999 9),173.5-2 776.0 μg/ml for promethazine hydrochloride(r=0.999 5) and 5.03-80.48 μg/ml for reserpine(r=0.999 1);limits of quantification were 18.80,14.92,25.70,12.20,4.99 ng,limits of detection were 5.54,4.50,8.04,3.66 1.50 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 1.0%;re-coveries were 98.20%-100.60%(RSD=0.89%,n=6),99.11%-100.28%(RSD=0.39%,n=6),99.07%-100.12%(RSD=0.38%, n=6),98.27%-99.70%(RSD=0.50%,n=6),97.41%-99.40%(RSD=0.88%,n=6),respectively. CONCLUSIONS:The meth-od is simple,accurate with high sensitivity and good reproducibility,and can be used for the simultaneous determination of vitamin B6,hydrochlorothiazide,rutin,promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet.关键词
高效液相色谱法/复方三嗪芦丁片/维生素B6/氢氯噻嗪/芦丁/盐酸异丙嗪/利血平/含量测定Key words
HPLC/Compound trizin and tutinum tablet/Vitamin B6/Hydrochlorothiazide/Rutin/Promethazine hydrochlo-ride/Reserpine/Content determination分类
医药卫生
