中国药房2016，Vol.27Issue(33)：4733-4734,4735,3.DOI:10.6039/j.issn.1001-0408.2016.33.42
HPLC法同时测定藏药独一味中山栀苷甲酯、木犀草素、芹菜素的含量
Simultaneous Determination of Dipsacoside B,Luteolin and Apigenin in Zang Medicine Lamiophlomis rotata by HPLC
乙永林 1孙连先2
作者信息
- 1. 连云港市疾病预防控制中心，江苏连云港 222003
- 2. 连云港市第二人民医院，江苏 连云港 222023
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of dipsacoside B,luteolin and apigenin in Zang medicine Lamiophlomis rotata. METHODS:HPLC was performed on the column of Diamonds C18 with mobile phase of acetoni-trile-0.1% phosphoric acid solution(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 238 nm for dipsaco-side B,348 nm for luteolin and apigenin,column temperature was 25 ℃,and injection volume was 10 μl. RESULTS:The linear range was 3.08-308μg/ml for dipsacoside B(r=0.999 8),3.72-372μg/ml for luteolin(r=0.999 9)and 2.92-292μg/ml for apigen-in(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.38%-103.15%(RSD=1.85%,n=9),96.05%-102.66%(RSD=2.07%,n=9) and 95.49%-103.59%(RSD=2.18%,n=9). CONCLUSIONS:The method is simple with good precision,stability and reproducibility,and can be used for the simultaneous determination of dip-sacoside B,luteolin and apigenin in Zang medicine L. rotata.关键词
独一味/山栀苷甲酯/木犀草素/芹菜素/含量/高效液相色谱法Key words
Lamiophlomis rotata/Dipsacoside B/Luteolin/Apigenin/Content/HPLC分类
医药卫生引用本文复制引用
乙永林,孙连先..HPLC法同时测定藏药独一味中山栀苷甲酯、木犀草素、芹菜素的含量[J].中国药房,2016,27(33):4733-4734,4735,3.
