中国药业2016，Vol.25Issue(21)：7-9,10,4.
当归补血汤对大鼠肾缺血－再灌注损伤后TLR4／NF－κB信号通路的影响
Influence of Danggui Buxue Decoction on TLR4/NF-κB Signal Pathway of Rats after Renal Ischemia Reperfusion Injury
摘要
Abstract
Objective To investigate the influence of Danggui Buxue Decoction on TLR4/NF-κB signal pathway of rats after renal is-chemia reperfusion injury ( RIRI ) , to provide reference for interpretation of its mechanism and intervention. Methods 45 SD rats were randomly divided into the Sham group, IR group, and IR+DG group, 15 rats in each group. 5 d before experiment, rats in Sham group and IR group were orally once a day with normal saline by 10 mL/ (kg·d), and rats in IR+DG group were orally once a day with Danggui Buxue Decoction by 10 mL/ (kg·d). All rats were resected right kidney, rats in Sham group were dissociated left renal pedi-cle, without clamping left renal artery, and rats in IR group and IR+DG group were all given RIRI model preparation. After experi-ment, serum creatinine ( Cr ) and urea nitrogen ( BUN ) , renal histology and renal tubule score, TLR4 mRNA and NF-κB p65 mRNA in renal tissue were compared. Results All groups completed operation according to predetermined plan;IR group and IR+DG group suc-cessfully completed RIRI model preparation. Serum Cr and BUN level, TLR4 mRNA and NF-κB p65 mRNA in renal tissue of IR group and IR+DG group were both higher than that in sham group ( t=3. 72-7. 05, P < 0. 05 or P < 0. 01 ) , and serum Cr and BUN level, TLR4 mRNA and NF-κB p65 mRNA in renal tissue of IR+DG group were lower than that in IR group ( t=4. 16-5. 37, P < 0. 05 ) . Sham group's kidney showed normal structure, and there were some inflammatory reactions in IR group and IR+DG group, yet IR+DG group's inflammatory reaction were slighter than that in IR group. Renal tubules score in IR group and IR+DG group were both higher than that in sham group ( tIR=9. 02, tIR+DG=6. 21, P < 0. 01 ) , yet IR+DG group's renal tubules score were lower than that in IR group ( tIR=5. 15, P < 0. 05 ) . Conclusion It may mediate downstream cascade inflammatory immune response by TLR4/NF-κB signal pathway after RIRI in rats. Danggui Buxue Decoction pretreatment may relieve inflammatory response during RIRI process by inhibiting LR4/NF-κB signal pathway, thus has positive protection to RIRI.关键词
缺血-再灌注损伤/信号转导/免疫炎性反应/当归补血汤/干预/大鼠Key words
ischemia reperfusion injury/signal transduction/inflammatory immune response/Danggui Buxue Decoction/intervention/rat分类
医药卫生引用本文复制引用
刘福和,倪文娟,王国康,徐权毅..当归补血汤对大鼠肾缺血－再灌注损伤后TLR4／NF－κB信号通路的影响[J].中国药业,2016,25(21):7-9,10,4.基金项目
浙江省教育厅科研项目，项目编号Y201534611。 （）
