中山大学学报（自然科学版）2016，Vol.55Issue(6)：125-131,7.DOI:10.13471/j.cnki.acta.snus.2016.06.019
Ca2＋对希瓦氏菌MR－1胞外多糖的影响
Effect of Ca2+on the extracellular polysaccharide of Shewanella oneidensis MR-1
摘要
Abstract
Shewanella is a gramnegative soil iron reducing bacteria,which has extracellular electrontransfer ability.The composition of the extracellular polymer is one of the important factors that can directly influence the electron transfer effect.Extracellular polysaccharide (EPS)is an important component of extracellular polymers.The effect of different concentrations of CaCl2 (0,0.7,1.4,2.1 and 5.0 mmol/L)on the EPS of Shewanella oneidensis MR -1 was herein studied by phenol sulfuric acidmethod combined with spectrophotometry,chromatography and mass spectrometry.The results showedthat the amount of EPS would vary with the Ca2 + concentration.Maximum EPS production of 0.3 g /mLwas obtained in the culture medium with 2.1 mmol/L of Ca2 + concentration,which was 5 times greaterthan that obtained without Ca2 +.The amount of EPS obtained with 1.4 mmol/L Ca2 + concentration was 0.08 g/mL,which was nearly the same to that obtained without Ca2 +.The concentration of Ca2 + in themedium did not significantly influence the structure of EPS produced by MR -1.EPS had a repeating u nit with a [→3) -α-D-Manp (1→]backbone chain.The results of this study provide reference da-ta for the further study of Shewanella extracellular polymers.关键词
Shewanella oneidensis MR-1/钙离子/胞外多糖/甘露糖Key words
Shewanella oneidensis MR-1/exopolysaccharides/Ca2+/mannose分类
生物科学引用本文复制引用
唐连凤,周丽华,孙丽华,宋少云..Ca2＋对希瓦氏菌MR－1胞外多糖的影响[J].中山大学学报（自然科学版）,2016,55(6):125-131,7.基金项目
国家自然科学基金资助项目（41301264）；广东省自然科学基金资助项目 （）
