作物学报2016，Vol.42Issue(12)：1754-1763,10.DOI:10.3724/SP.J.1006.2016.01754
水稻温敏型叶片白化突变体tsa1的表型鉴定和基因定位
Phenotypic Characterization and Gene Mapping of a Thermo-sensitive Albino Leaf Mutanttsa1 in Rice
摘要
Abstract
A thermo-sensitive albino leaf mutanttsa1 was obtained from Ningjing 36 induced by ethyl methane sulfonate (EMS). The mutant developed albinotic leaves under low temperature (20-24°C), and the color of mutant leaves was comparable with the wild-type under high temperature (28-32°C). Compared with wild type, the chlorophylla and carotene contents of mutant albino-tic leaves obviously decreased under low temperature condition. With microscope observation, the quantity of normal chloroplast significantly decreased, besides, there were a large number of small abnormal chloroplasts in albinotic leaves. The abnormal chloroplast in albinotic tissue of mutant had no obvious thylakoid stack under transmission electron microscopy. These results indicate thattsa1 mutant has defect on chloroplast development under low temperature. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using 368 reces-sive individuals from F2 population oftsa1/Nanjing 11, the target gene was finally located in a 163 kb region on the chromosome 5. This study provides a basis for map-based cloning and functional analysis of the target gene.关键词
水稻(Oryza sativa.L)/温敏型/白化/突变体/叶绿体发育Key words
Rice (Oryza sativaL.)/Thermo-sensitive/Albino/Mutant/Chloroplast development引用本文复制引用
刘钰龙,刘峰,周坤能,苏晓妹,方先文,张云辉,鲍依群..水稻温敏型叶片白化突变体tsa1的表型鉴定和基因定位[J].作物学报,2016,42(12):1754-1763,10.基金项目
本研究由国家自然科学基金项目(31401036),江苏省自然科学基金项目(BK20130706),江苏省农业科技自主创新资金[CX(14)5005],江苏省农业科学院基本科研业务专项[ZX(15)4015]和安徽省自然科学基金项目(1408085MKL62)资助。This study was supported by the National Natural Science Foundation (31401036), the Natural Science Foundation of Jiangsu Province (BK20130706), the Jiangsu Province Agricultural Science and Technology Independent Innovation Foundation [CX(14)5005], the Jiangsu Academy of Agricultural Sciences Fundamental Research Projects [ZX(15)4015], and the Natural Science Foundation of Anhui Province (1408085MKL62) （31401036）
