作物学报2016，Vol.42Issue(12)：1827-1833,7.DOI:10.3724/SP.J.1006.2016.01827
SA、MeJA和ACC处理对甘蓝型油菜叶角质层蜡质组分、结构及渗透性的影响
Effects of SA, MeJA, and ACC on Leaf Cuticular Wax Constituents, Structure and Permeability inBrassica napus
摘要
Abstract
Cuticular waxes are related to plant adaptation to environment stress. In the current study,Brassica napus cv. Zhong-shuang 11 grown in the soil treated with adding solutions of SA (200μmol L–1), MeJA (100μmol L–1), and ACC (200μmol L–1) wax, were sampled at the five-leaf stage to clarify the effects of SA, MeJA and ETH on adjusting plant cuticular wax deposition. The leaves were used to analyze the amounts of total cuticular wax and wax constituents, wax crystal structure, and cuticular per-meability. The amounts ofn-alkanes, secondary alcohols, ketones, aldehydes and total cuticular waxes increased significantly when compared with the control at seven days after MeJA treatment, whereas the amounts of all wax constituents and total cu-ticular waxes significantly decreased at 14 days after MeJA treatment. The application of SA and ACC had no significant influ-ence on cuticular wax deposition at 7 and 14 days after treatments, except for an amount reduction of primary alcohol, aldehyde and unknown constituents for SA treatment at 14 days after treatment. At 21 days after SA, MeJA, and ACC application, the amounts of total cuticular wax,n-alkanes, ketones and aldehyde significantly increased. C29 n-alkane, C29 ketone, and C30 al-dehyde were the main cuticular wax constituents induced by SA, MeJA, and ACC application, implying thatn-alkane, ketone and aldehyde might be related to the resistance to stresses induced by these signal molecules. Scan electric microscope analysis in-dicted that the rod-shape structure of cuticular wax in leaf surface reduced and some rods melted under SA treatment. MeJA and ACC application increased the distribution density of wax crystalloids. The cuticular wax deposition and crystal structure altera- tion reduced the cuticular permeability and delayed the leaf water loss. The specific increase of C29n-alkane might be the main reason for reducing leaf water loss inB. napus.关键词
甘蓝型油菜/角质层蜡质/水杨酸(SA)/茉莉酸甲酯(MeJA)/乙烯(ETH)Key words
Brassica napus L/Cuticular wax/Salicylic acid/Methyl jasmonic acid/Ethylene引用本文复制引用
李帅,赵秋棱,彭阳,徐熠,李加纳,倪郁..SA、MeJA和ACC处理对甘蓝型油菜叶角质层蜡质组分、结构及渗透性的影响[J].作物学报,2016,42(12):1827-1833,7.基金项目
本研究由中央高校基本科研业务费专项(XDJK2014B037),重庆市基础与前沿研究计划项目(cstc2016jcyjA0170)和国家自然科学基金项目(31000122)资助。This study was supported by the Fundamental Research Funds for the Central Universities (XDJK2014B037), the Chongqing Basic and Advanced Research Project (cstc2016jcyjA0170), and the National Natural Science Foundation of China (31000122) （XDJK2014B037）
