北京林业大学学报2016，Vol.38Issue(11)：118-124,7.DOI:10.13332/j.1000-1522.20150484
黑果花楸花色苷提取、纯化工艺研究
Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa
摘要
Abstract
Anthocyanins were extracted from Aronia melanocarpa with water, 50% -100% ethanol and 50% -100% methanol. The results showed that the optimal yield of anthocyanins was with 80% ethanol. Four single factor experiments were designed for extracting time, solid-liquid ratio, pH and temperature. Based on the single factor experiment analysis, the mathematical model was established by Box-Behnken response surface design. The results showed that the optimum extracting conditions were:extracting time 110 min, pH 2, extracting temperature 34 ℃, solid-liquid ratio 1: 16. 5. Under these conditions, anthocyanins yield reached 6. 648 mg/g. AB-8 was chosen as the optimal resin after the static adsorption and desorption testing. The optimum purification conditions were 2 mg/mL of solution concentration, 1:25 of diameter to length, 2. 2 BV, pH 2 and 80% ethanol with the elution flow rate of 2. 0 BV/h, the anthocyanins purity increased 11. 5 times.关键词
黑果花楸/响应面优化/花色苷/提取纯化工艺Key words
Aronia melanocarpa/response surface methodology/anthocyanins/extraction and purification process分类
农业科技引用本文复制引用
郑悦,王萍..黑果花楸花色苷提取、纯化工艺研究[J].北京林业大学学报,2016,38(11):118-124,7.基金项目
卓越农林人才教育培养计划改革试点项目(41110211)。 （41110211）
