福建农林大学学报（自然科学版）2016，Vol.45Issue(6)：668-672,5.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.06.010
高效液相色谱-电化学法检测蜜蜂脑部单胺类神经递质及其酸性代谢产物含量
Applications of high performance liquid chromatography-electrochemical method in content determination of monoamine neurotransmitters and their metabolites in honeybee brains
摘要
Abstract
A method based on high liquid chromatography-electrochemical ( HPLC-ECD) was established to simultaneously detect neuro substances in honeybee brains, which included levodopa ( l-DOPA) , norepinephrine ( NE) , 3,4-dihydroxyphenylacetic acid (DOPAC), dopamine (DA), 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and serotonin (5-HT). Mono-amine transmitters and their metabolites were isolated by an Acclaim C18 column ( 2. 2 μm, 2. 1 × 100 mm) in 38 ℃ in mobile phase, which consisted of NaH2 PO4 , sodium 1-octanesulfonate, citric acid, EDTA and acetonitrile, at a flow rate of 0. 2 mL•min-1 . After proteins of honeybee brains were precipitated by mixture of perchloric acid, Na2 S2 O5 and EDTA, 10 μL supernatant was filtrated with 0.22 μm membrane, and followed by being detected at 300 mV. Results showed that the correlation coefficient of each standard curve was over 0.997, with average relative standard differences ( RSD) being less than 5.7% in inter-or intra-day as-say for all neuro substances. Average recovery rate was higher than 90%. Contents of l-DOPA, NE, DOPAC, DA and 5-HIAA in A-pis mellifera brains were (0.232±0.107), (1.288±0.630), (2.555±1.201), (0.574±0.233), (0.393±0.123) ng•brain-1, re-spectively. The corresponding results in Apis cerana brains were (0.331±0.162), (0.894±0.395), (2.819±0.974), (0.636± 0.255), (0.353±0.163) ng•brain-1, respectively.关键词
神经递质/液相色谱/电化学/蜜蜂/脑Key words
neurotransmitter/HPLC/ECD/honeybee/brain分类
农业科技引用本文复制引用
赵晓蒙,苏松坤..高效液相色谱-电化学法检测蜜蜂脑部单胺类神经递质及其酸性代谢产物含量[J].福建农林大学学报（自然科学版）,2016,45(6):668-672,5.基金项目
国家现代农业产业技术体系(蜜蜂)项目( CARS-45) （蜜蜂）
