分析化学2016，Vol.44Issue(12)：1801-1807,7.DOI:10.11895/j.issn.0253-3820.160344
基于MspA蛋白质纳米孔传感器的主客体化学研究
A Single-Molecule Mycobacterium Smegmatis Porin A Protein Nanopore Sensor for Host-guest Chemistry
段静 1卓莎 1姚付军 1张亚妮 2亢晓峰1
作者信息
- 1. 西北大学化学与材料科学学院，西安710127
- 2. 西北大学生命科学学院，西安710069
- 折叠
摘要
Abstract
A mycobacterium smegmatis porin A ( MspA) protein nanopore was prepared by E. coil prokaryotic expression system. The extraction experiments of MspA nanopore using surfactants showed that an extraction agent concentration of 0 . 5%, a temperature of 90℃ and an extraction time of 30 min were the optimum extraction conditions. The interactions of MspA nanopore with a variety of cyclodextrins were examined at single-molecule level. The interaction between MspA and Per-6-amino-β-cyclodextrin ( am7-β-CD ) was stronger and consistent with blocking platform. Its retention time in the MspA nanopore decreased with the increasing voltage, whereas the blocking current was almost not affected by voltage. So the am7-β-CD was selected as a non-covalent adapter and lodged within single MspA nanopore to study the single-molecule host-guest chemistry. After adding am7-β-CD to form a first blocked platform, a small concentration of 50 μmol/L for the amantadine hydrochloride showed a second blocked platform and its residence time decreased with the increasing voltage. This new protein nanopore sensor can be used as a single-molecule detector and identifier for small organic molecules, which will greatly broaden the application range of MspA nanopore.关键词
Mycobacterium smegmatis porin A/纳米通道/单分子检测/环糊精/盐酸金刚烷胺Key words
Mycobacterium smegmatis porin A/Nanopore/Single-molecule detection/Cyclodextrin/1-Amantadine hydrochloride引用本文复制引用
段静,卓莎,姚付军,张亚妮,亢晓峰..基于MspA蛋白质纳米孔传感器的主客体化学研究[J].分析化学,2016,44(12):1801-1807,7.
