分析化学2016，Vol.44Issue(12)：1820-1827,8.DOI:10.11895/j.issn.0253-3820.160449
基于磁性金属有机框架化合物-适配体探针的氯霉素仿生比色传感器研究
A Colorimetric Bionic Aptasensor Base on Magnetic Metal Organic Framework Compound-Aptamer Probe for Specific Detection of Chloramphenicol
摘要
Abstract
A colorimetric bionic aptasensor based on magnetic metal organic framework ( MOF ) compound-aptamer bionic probe was designed for detecting chloramphenicol ( CAP ) . In this assay, the aptamer was labeled on the magnetic beads as capture probe and its complementary strand ( cDNA ) was immobilized on MOF as the nanotracer composite probe, and the ferromagnetic biomimetic composite probe was obtained by hybridizing the capture probe with the tracer. When the composite probe solution was incubated with CAP, the aptamer on the capture probes preferentially combines with CAP, and the signal tag was substituted into supernatant after magnetic separation. The supernatant containing numerous MOF could efficiently catalyze tetramethylbenzidine ( TMB )-H2 O2 solution for the color development quantified by ultraviolet-visible spectroscopy. Under the optimal reaction conditions, the experimental results showed the CAP detection owned a linear range of 0 . 001-10 ng/mL and limit of detection was 0 . 3 pg/mL ( S/N=3 ) . The recoveries of standard addition in the actual samples were 86. 9% -93. 5% with no interference of other antibiotics. Besides, the results of this method for CAP detection in the milk samples agreed well with the ELISA results. Compared with the enzyme labeled probe, the non-enzyme labeled bionic probe using the magnetic separation greatly simplifies the pre-processing step, and it has many advantages such as high catalytic activity and low cost, and can be applied to the field detection of CAP in food with rapidity, high specificity and high sensitivity.关键词
磁性金属有机框架化合物/磁性捕获探针/氯霉素/适配体Key words
Magnetic metal organic framework compound/Magnetic capture probe/Chloramphenicol/Aptamer引用本文复制引用
吴彩叶,缪养宝,朱云云,干宁,欧昌荣,曹锦轩..基于磁性金属有机框架化合物-适配体探针的氯霉素仿生比色传感器研究[J].分析化学,2016,44(12):1820-1827,8.基金项目
本文系国家自然基金科学项目(Nos.31271890,31501519)、浙江省和宁波自然科学基金(Nos. LY 15B050002,LY 16B050003,2016A610084)、“水产冶浙江省重中之重学科开放基金( No. xkzsc1428)、宁波大学科研基金(No. xkzsc1524)资助 （Nos.31271890,31501519）
