分析化学2016，Vol.44Issue(12)：1892-1899,8.DOI:10.11895/j.issn.0253-3820.160296
基于废弃固定化酶再利用的高容量强阳离子交换树脂及其对溶菌酶的吸附性能
High-Capacity Strong Cation Exchange Resin Based on Reutilization of An Inactivated Immobilized Enzyme and Its Adsorption Properties for Lysozyme
摘要
Abstract
A new cation exchange resin was prepared based on surface-initiated atom transfer radical polymerization (SI-ATRP) of poly (sodium 4-styrene sulfonate) (PNASS) at 30℃ for 3 h from inactivated immobilized enzymes. The cation exchange resin was characterized by Fourier transform infrared spectrometry ( FT-IR) . Lysozyme was selected as a model protein to evaluate the adsorption performance of the as-prepared cation exchange resin. The effects of protein concentration, ionic strength, organic solvent, contacting time and temperature on protein adsorption were investigated. The results showed that the cation exchange resin exhibited very good performance for the adsorption of lysozyme with a high capacity ( 240 mg/g ) at room temperature and fast speed ( within 30 min ) , which were much better than those reported in previously reported works. The adsorption isotherms could be better fitted by Langmuir model than Freundlich model and the adsorption process followed the pseudo-second-order kinetic model.关键词
离子交换树脂/固体废弃物/表面引发-原子转移自由基聚合/吸附/溶菌酶Key words
Ion-exchange resin/Solid wastes/Surface-initiated atom transfer radical polymerization/Adsorption/Lysozyme引用本文复制引用
袁景香,王超展,卫引茂..基于废弃固定化酶再利用的高容量强阳离子交换树脂及其对溶菌酶的吸附性能[J].分析化学,2016,44(12):1892-1899,8.基金项目
本文系国家自然科学基金项目(Nos.21575114,21475104)资助 （Nos.21575114,21475104）
