广西师范大学学报（自然科学版）2016，Vol.34Issue(3)：131-137,7.DOI:10.16088/j.issn.1001-6600.2016.03.019
沙田柚WRKY转录因子的克隆与序列分析
Cloning and Sequence Analysis of WRKY Transcription Factor from Citrus grandis var.Shatinyu Hort
摘要
Abstract
In this study,the transcriptome of the self-pollinated styles and cross-pollinated styles of Citrus grandis var.Shatinyu Hort were sequenced by high-throughput sequencing technology.WRKY transcription factor gene of Citrus grandis var.Shatinyu Hort was obtained through variance analysis method.It is 1 178 bp in length with an open reading frame (ORF)of 993 bp,encoding 330 amino acids with deduced molecular weight of 37.00 ku,and theoretical pI value of 5.79.Compared with un-pollinated styles,the expression (RPKM)of WRKY transcription factor gene is respectively 5.67,26.04 and 17.08 in 1,2,3 days cross-pollinated styles,and expression (RPKM)of the gene is respectively 15.67,14.96 and 3.89 in 1,2,3 days self-pollinated styles.The homology analysis of amino acid sequence indicated that the WRKY transcription factor protein shared higher homology with that of Citrus sinensis (98%) and Citrus japonica (97%).Phylogenetic analysis revealed that WRKY transcription factor gene showed closer kinship with that of Citrus sinensis and Citrus_japonica, indicating that they belong to the same evolutionary branch.关键词
沙田柚/WRKY/转录因子/自交不亲和性Key words
Citrus grandis var.Shatinyu Hort/WRKY/transcription factor/self-incompatibility分类
农业科技引用本文复制引用
张渝,刘玉洁,郭丹妮,覃信梅,韩愈,李惠敏,秦新民..沙田柚WRKY转录因子的克隆与序列分析[J].广西师范大学学报（自然科学版）,2016,34(3):131-137,7.基金项目
国家自然科学基金资助项目(31360477) （31360477）
广西教育厅项目基金资助(2013YB036) （2013YB036）
