湖南农业大学学报（自然科学版）2016，Vol.42Issue(6)：592-600,9.DOI:10.13331/j.cnki.jhau.2016.06.003
不同产区加工的茯砖茶中“金花”菌的分离及分子鉴定
Isolation and molecular identification of “Jinhua” fungi on the Fuzhuan tea produced in different regions
摘要
Abstract
In order to study the differences of the “Jinhua” fungi from the fuzhuan tea (FZT)produced in different regions, 9 FZT samples, which were processed with the same standard of raw dark teas from Shanxi, Hunan, Zhejiang at the same season, were used as materials. The “Jinhua” fungi obtained from the FZT samples were cultured with different mediums. The species of the tested fungi were identified by the morphology of colony observed with microscope and DNA sequencing. The results revealed that 13 strains of “Jinhua” fungi were obtained from the 9 FZT samples. All the fungi had similar morphological characteristics to theEurotiumsp., while the strains of G9 and G10 were obviously different from the other “golden flora” fungi. By amplifying and sequencing the specific segments sequences of the ITS andβ–tubulinand, comparing the homology sequence, analyzing phylogenetic trees, and referring to Hubka’s latest classification system ofAspergillus,the dominant fungi obtained from the FZT produced in different regions were identified as A. cristatus and the non-dominant fungi strains of G9, G10 were identified asA. chevalieri.关键词
茯砖茶/“金花”菌/分子鉴定/分类Key words
Fuzhuan tea/“Jinhua” fungi/molecular identification/taxonomy分类
轻工纺织引用本文复制引用
赵仁亮,吴丹,姜依何,朱旗..不同产区加工的茯砖茶中“金花”菌的分离及分子鉴定[J].湖南农业大学学报（自然科学版）,2016,42(6):592-600,9.基金项目
国家自然科学基金项目(31571802)；湖南省教育厅重点项目(14A066) （31571802）
