湖南农业大学学报（自然科学版）2016，Vol.42Issue(6)：601-607,7.DOI:10.13331/j.cnki.jhau.2016.06.004
蛹虫草中蓝光受体基因Cmwc-1和Cmwc-2的表达特性分析
Expression characteristics of blue light receptorCmwc-1and Cmwc-2 gene fromCordyceps militaris
摘要
Abstract
ORF sequence of geneCmwc–1andCmwc–2,the blue light receptor, were cloned from fungusCordyceps militaris by approach of RT–PCR. There were 1 PAS functional domains and 1 zinc finger functional domain at putative CmWC–2 amino acid sequence in Cmwc–2ORF. The real-time PCR results showed that the translation of gene Cmwc–1 andCmwc–2 reached their peaks in 6 h and 2 h, respectively. The relative transcription level of gene Cmwc–1 and Cmwc–2 were quite different according to their growing stages. The relative transcription level ofCmwc–1 was mainly distributed at the middle and lower section of fruiting body, and rarely spotted at the top after 50 d inoculation, while, it was mainly at the top and the middle section forCmwc–2.There were little relative transcription level ofCmwc–1 and Cmwc–2in abnormal fruiting body.关键词
蛹虫草/蓝光/蓝光受体/基因Cmwc-1/基因Cmwc-2Key words
Cordyceps militaris/blue light/blue light receptor/Cmwc-1/Cmwc-2分类
生物科学引用本文复制引用
李琴,毛玉梅,付鸣佳,沈俊良,金华燕,钟雪晴..蛹虫草中蓝光受体基因Cmwc-1和Cmwc-2的表达特性分析[J].湖南农业大学学报（自然科学版）,2016,42(6):601-607,7.基金项目
国家自然科学基金项目(31060004；31260010) （31060004；31260010）
