湖南农业大学学报（自然科学版）2016，Vol.42Issue(6)：615-621,7.DOI:10.13331/j.cnki.jhau.2016.06.006
外源一氧化氮对铝胁迫下烟草叶片光能利用和光保护系统及活性氧代谢的影响
Effects of nitric oxide on light energy utilization, photoprotective systems and reactive oxygen metabolism in leaves of tobacco seedlings under aluminum stress
摘要
Abstract
The effects of sodium nitroprusside(SNP, an exogenous NO donor)on aluminum(Al)toxicity in tobacco were studied by investigating plant growth, reactive oxygen content, photosynthesis, chlorophyll fluorescence characteristics, photorespiration and antioxidative enzymes in leaves of tobacco cultivar Yunyan 100(Al-tolerant)and Yunyan 105(Al-sensitive)with solution culture method. The results showed that under Al stress, the leaf chlorophyll content, net photosynthetic rate(Pn), maximal photochemical efficiency(Fv/Fm), quantum yield of electron transport at PSII(FPSI)and coefficient of photochemical quenching(qP) were dramatically decreased, which increased excess energy of PSII photochemistry leading to higher accumulation of O2·andH2O2, and lower plant biomass in both genotypes. Under Al stress, application of exogenous NO dramatically increased the leaf chlorophyll content,Pn,Fv/Fm,FPSI,qP and plant biomass, decreased excess energy of PSII photochemistry and accumulation of O2·andH2O2,the effects were more predominantly in Yunyan 105 than in Yunyan 100. Furthermore, the coefficient of non-photochemical quenching, photorespiration rate, and activities of superoxide dismutase and ascorbate peroxidase were increased in both genotypes in the presence of NO under Al stress. These results indicated that application of exogenous NO could enhance the tolerance of tobacco to Al stress mainly by increasing of the photochemical reaction capacity, heat dissipation, photorespiration and activities of antioxidative enzymes, which prevents or eliminates ROS accumulation, and the effect is greater in the Al-sensitive genotype than in the Al-tolerant genotype.关键词
烟草/铝胁迫/一氧化氮/活性氧/光合作用/叶绿素荧光/光呼吸/抗氧化酶Key words
tobacco/aluminum stress/nitric oxide/reactive oxygen species/photosynthesis/chlorophyll fluorescence/photorespiration/antioxidant enzyme分类
农业科技引用本文复制引用
刘强,贺根和,柳正葳,苏金雄..外源一氧化氮对铝胁迫下烟草叶片光能利用和光保护系统及活性氧代谢的影响[J].湖南农业大学学报（自然科学版）,2016,42(6):615-621,7.基金项目
国家自然科学基金项目(31260498)；江西省教育厅项目(GJJ12466) （31260498）
