湖南农业大学学报（自然科学版）2016，Vol.42Issue(6)：641-646,6.DOI:10.13331/j.cnki.jhau.2016.06.011
赤眼鳟TRAF6基因的cDNA克隆及其应对GCRV的免疫表达特性
Molecular cloning and expression characteristics response to GCRV ofTRAF6 inSqualiobarbus curriculus
摘要
Abstract
In order to investigate whether tumor necrosis factor-associated factor 6 gene involved inantiviral immune response, the full-length cDNA ofTRAF6inSqualiobarbus curriculus was cloned by using RACE–PCRs method. The full-length cDNA ofTRAF6 is 2 621 bp including a 5′–terminal untranslated region of 50 bp, a 3′–terminal untranslated region of 942 bp and an open reading frame of 1 629 bp encoding a polypeptide of 542 amino acid residues. The putative isoelectric point and molecular weight ofTRAF6 was 6.01 and 61 670, respectively. The protein encoded by this gene includes one ring domain, two zinc fingers, one coiled-coil region, and one MATH domain. Phylogenetic analysis showed that theTRAF6 was the most homogeneous toMegalobrama amblycephala. The expression ofTRAF6could be detected in all the 12 tested tissues by RT–PCR, which thehighest expressed in the liver andthe lowest expressed in midgut.After injection with grass carp reovirus (GCRV), theTRAF6expression level was up and down regulated in both spleen and head kidney, and reached the peak at 24 h, respectively. The results suggest thatTRAF6 gene might play important roles in the immune response ofSqualiobarbus curriculus against GCRV.关键词
赤眼鳟/肿瘤坏死因子受体相关因子6基因/草鱼呼肠孤病毒(GCRV)Key words
Squaliobarbus curriculus/tumor necrosis factor receptor-associated factor 6 gene (TRAF6)/grass carp reovirus(GCRV)分类
生物科学引用本文复制引用
陈开健,王静安,刘巧林,王荣华,徐莹,赵鑫,肖调义..赤眼鳟TRAF6基因的cDNA克隆及其应对GCRV的免疫表达特性[J].湖南农业大学学报（自然科学版）,2016,42(6):641-646,6.基金项目
国家自然科学基金青年项目(31402289)；国家自然科学基金项目(31272652；31572615) （31402289）
