湖南农业大学学报（自然科学版）2016，Vol.42Issue(6)：654-657,4.DOI:10.13331/j.cnki.jhau.2016.06.013
脱脂松仁粕中球蛋白的提取工艺及其SDS-PAGE分析
Optimization of extraction globulin from defatted pine nut meal and analysis of SDS-PAGE
摘要
Abstract
Defatted pine nut meal was used as material, four single factors of NaCl extraction solvent (0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 3.0%), ratio of material to solvent (1∶10, 1∶15, 1∶20, 1∶25, 1∶30, and 1∶35), extraction time(30, 60, 90, 120, 150, and 180 min) and extraction temperature (40, 45, 50, 55, 60, and 65℃ )were designed to extract globulin by the method of Osborne and to improve extraction process by response surface method and to analyze relative molecular mass of globulin by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE). The result showed that the optimum extraction conditions were 2.5% of NaCl, extraction at 56℃ for 150 min with ratio of material to solvent 1∶15, the extraction ratio of globulin was up to 15.32%, and the relative molecular mass of the proteins were 41 320, 27 520, 21 690, 16 310, 15 180.关键词
脱脂松仁粕/球蛋白/提取工艺/十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)Key words
defatted pine nut/globulin/extraction process/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)分类
轻工纺织引用本文复制引用
包怡红,赵楠..脱脂松仁粕中球蛋白的提取工艺及其SDS-PAGE分析[J].湖南农业大学学报（自然科学版）,2016,42(6):654-657,4.基金项目
中央高校科研业务费专项(2572014EA02)；哈尔滨市科技创新人才项目(2015RAXXJ010) （2572014EA02）
