海洋科学2016，Vol.40Issue(9)：1-8,8.DOI:10.11759/hykx20160408003
海湾扇贝维甲酸受体（RXR）cDNA克隆与表达分析
Molecular cloning and expression of the retinoid X receptor gene inArgopecten irradians
摘要
Abstract
The retinoid X receptor (RXR) is a member of the nuclear family of receptors. It has been proven that RXR plays a crucial role in regulating growth, development, metabolism, and other physiological process in verte-brates. Using the RACE technique, the cDNA sequence ofArgopecten irradiansRXR (AiRXR) was cloned. The ORF of AiRXR was 1338 bp. Two isoforms were confirmed as AiRXRα and AiRXRβ with a difference of four amino acids in the T-box. The homologous analysis of the amino acids showed that AiRXRs share a high degree of similarity with the RXRs ofHomo sapiens andChlamys farrerii. The results of qRT-PCR illustrated that the AiRXR genes are expressed in multiple tissues (mantles, gills, gonads, and adductor muscles) throughout gonadal devel-opment, indicating that AiRXRs act as one of the signal molecules involved in multiple physiological processes. The highest level of AiRXR expression was observed at the proliferation stage of gonadal development, implying that AiRXR may be involved in the regulation of gonadal development and differentiation.关键词
海湾扇贝(Argopecten irradians)/RXR基因/组织表达/性腺发育Key words
Argopecten irradians/AiRXR gene/tissue expression/gonadal development分类
生物科学引用本文复制引用
张瑞,阙华勇,丛日浩,李莉,张国范..海湾扇贝维甲酸受体（RXR）cDNA克隆与表达分析[J].海洋科学,2016,40(9):1-8,8.基金项目
国家科技部高技术研发计划课题(863计划,2012AA10A410) （863计划,2012AA10A410）
贝类产业技术体系项目(CARS-48) （CARS-48）
中国科学院科技服务网络计划项目(KFJ-EW-STS-060)[Foundation：The National High Technology Research and Development Program,863 Program, No.2012AA10A410 （KFJ-EW-STS-060）
The Earmarked Fund for Modern Agro-industry Technology Research System, No.CARS-48 （）
Sci-ence and Technology Service Network Initiative No.KFJ-EW-STS-060] （）
