海洋科学2016，Vol.40Issue(9)：9-17,9.DOI:10.11759/hykx20150305002
大菱鲆家系选育二代7种免疫因子的分析
Analysis of seven immune-related genes in selective second filial families (F2) of turbot (Scophthalmus maximus)
摘要
Abstract
For breeding turbot group with high survival rates and resistance toVibrio anguillarum, 30 selective second filial families (F2) with a high survival rate and one common culture group of turbot (Scophthalmus maximus L.) were infected with V. anguillarum. Six F2 and one control groups were picked out as samples for detecting the following immune responses. Gene expressions of seven immune-related factors (lysozyme, HSP90, HSP70, IgM, hepcidin, C-type lectin, and lily-type lectin) were analyzed in turbot livers, spleens, and head kidneys using RT-PCR. Correlation analysis between the expression of these immune genes before infection and survival rate after challenge assays (period of 14 days) was carried out using SPSS 16.0 software. Results showed that regardless of samples infected withV. anguillarum, the expression of immune-related genes in the six F2 populations was generally stronger than that in the control and the expression status of most immune genes was higher in the seven experimental groups withoutVibrio stress thanin the injected groups. The expression of the remaining six immune genes, except hepcidin, beforeVibrio infection showed a significantly positive correlation with survival rate in livers (P < 0.05) and the gene expression of lysozyme and IgM exhibited a highly significant positive correlation (P< 0.01). Gene expressions of hepcidin, HSP70, and HSP90 showed a highly significant positive correlation with survival rate in spleens (P< 0.01); the expression of HSP70 exhibited a significant positive correlation with survival rate in head kidneys (P< 0.05). Therefore, F2 selective families with a high survival rate could possess an outstanding resistance againstV. anguillarum and the seven immune-related genes could play important roles in bacterial resistance of turbot. We obtained a selective family (No. 23) with a strong resistance againstV. anguillarum, which could guide selection of turbot strains with disease-resistance trait in the future and provide a reference and theoretical basis for turbot breeding.关键词
大菱鲆(Scophthalmus maximus)/鳗弧菌(Vibrio anguillarum)/选育家系/免疫因子/RT-PCRKey words
Scophthalmus maximus/Vibrio anguillarum/selective second filial families/immune-related gene/RT-PCR分类
农业科技引用本文复制引用
田岳强,郭建丽,黄智慧,马爱军,王新安,杨志,曲江波..大菱鲆家系选育二代7种免疫因子的分析[J].海洋科学,2016,40(9):9-17,9.基金项目
国家863计划项目(2012AA10A408-8) （2012AA10A408-8）
现代农业产业技术体系建设专项基金共同资助(CARS-50-G01) （CARS-50-G01）
中国博士后科学基金项目(2015M572096) （2015M572096）
山东省自然科学基金项目(ZR2014CP001)[Foundation：National 863 Program (2012AA10A408-8) （ZR2014CP001）
Modern Agro-Industry Technology Research System (CARS-50-G01) （CARS-50-G01）
China Postdoctoral Science Foundation(2015M572096) （2015M572096）
the Shandong Provincial Natural Science Foundation (ZR2014CP001) （ZR2014CP001）
