军事医学2016，Vol.40Issue(11)：897-900,4.DOI:10.7644/j.issn.1674-9960.2016.11.011
敲低SET7慢病毒载体的构建及对乳腺癌细胞生长的影响
Construction of a lentiviral vector of SET7 small-interfering RNA and its effect on growth of breast cancer cells
摘要
Abstract
Objective To study the biological function of histone methyltransferase SET 7 in the development and pro-gression of breast cancer by constructing the lentiviral vector of SET 7 small-interfering RNA and investigating its effect on the growth of ZR75-1 breast cancer cells.Methods Based on human SET7 cDNA sequence, short hairpin RNA(shRNA) primers targeting SET7 were designed and cloned into the vector pSIH-H1-Puro.Lentivirus was obtained and infected with ZR75-1 breast cancer cells .Real-time PCR and Western blotting were performed to identify the lentivirus-mediated knockdown of SET7.Growth curves were used to study the effect of SET 7 knockdown on the growth of ZR 75-1 cells. Results DNA sequencing indicated that the SET 7 shRNA lentiviral vector was constructed successfully .Real-time PCR and Western blotting showed that SET7 shRNA could suppress SET7 gene expression, which could in turn markedly inhibit the growth of ZR75-1 cells.Conclusion The lentivirus-mediated SET7 shRNA is successfully constructed .Knocking down SET7 could suppress the growth of ZR75-1 cells.关键词
SET7基因/慢病毒载体/RNA干扰/乳腺肿瘤Key words
SET7 gene/lentivirus vector/RNA interference/breast neoplasms分类
医药卫生引用本文复制引用
周蕾,姜妮,王小利,刘婕,丁丽华..敲低SET7慢病毒载体的构建及对乳腺癌细胞生长的影响[J].军事医学,2016,40(11):897-900,4.基金项目
国家自然科学基金资助项目 （）
