南京工业大学学报（自然科学版）2016，Vol.38Issue(6)：130-134,5.DOI:10.3969/j.issn.1671-7627.2016.06.022
乳源β Casein 125肽的基本特征及促B淋巴细胞增殖作用
Basic characteristics of a human milk-derived peptide β-Casein-125 and its effect on B lymphocyte proliferation
摘要
Abstract
The biologic characteristics of β⁃Casein⁃125 peptide were analyzed by Uniprot, SABLE, ProtParam tool and other online databases. The content ofβ⁃Casein⁃125 in colostrum, transition milk and mature milk was mesured by mass spectrometry technology. Furthermore, the effect of β⁃Casein⁃125 peptide in B lymphocyte proliferation was evaluated by MTT assay. The online databases showed that stability coefficient, aliphatic index and grand average of hydropathicity of β⁃Casein⁃125 were 111�43, 111�43 and 0�471, respectively. It indicated that β⁃Casein⁃125 is a hydrophobic stable peptide. The content of β⁃Casein⁃125 was significantly decreased with lactation time, detected by mass spectrometry technology. Moreover, β⁃Casein⁃125 could across the cell membrane of mouse B lymphocytes, and promote their proliferation. β⁃Casein⁃125, a hydrophobic peptide with high stability, could promote lymphocyte proliferation. It was indicated thatβ⁃Casein⁃125 played a critial role in promoting the neonatal immune system maturation.关键词
母乳/β Casein 125肽/B淋巴细胞/增殖Key words
human milk/β-Casein-125 peptide/B lymphocyte/proliferation分类
生物科学引用本文复制引用
刘贵友,万俊,肖文..乳源β Casein 125肽的基本特征及促B淋巴细胞增殖作用[J].南京工业大学学报（自然科学版）,2016,38(6):130-134,5.基金项目
江苏省自然科学基金 （）
