生物技术通报2016，Vol.32Issue(12)：96-102,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.12.016
青藏高原植物手参的谱系地理学研究
Phylogeography of Gymnadenia conopsea from the Qinghai-Tibet Plateau
摘要
Abstract
Chloroplast DNA(rbcl and psbA-trnH)of Gymnadenia conopsea(G. conopsea)from the Qinghai-Tibet Plateau was used for phylogeography research,aiming at revealing the distribution pattern and evolutional process of genetic lineage of G. conopsea. Eleven haplotypes were identified in 199 samples of 10 G. conopsea populations. The analysis of molecular variance(AMOVA)showed 65.93% of the genetic variance was from inter-populations and FST value of 0.659(P<0.01)also indicated that there was significant genetic differentiation between different populations. The genetic differentiation coefficients NST(0.398)< GST(0.626)implied no significant phylogeographic structure for G. conopsea populations in the Qinghai-Tibet Plateau. Mismatch analysis of multi-peak and Tajima’s D neutral test(-1.455, P>0.1)demonstrated G. conopsea populations had not experienced abrupt expansion recently. Phylogenetic analysis and divergence time estimation showed that G. conopsea in the Qinghai-Tibet Plateau originated indigenously,haplotypes started to differentiate 19.08 million years ago(Mya)in Miocene,indicating that the genetic differentiation of G. conopsea was related to the uplift of the Qinghai-Tibetan Plateau,and the genetic distribution pattern of G. conopsea was mostly formed before the Quaternary glaciation.关键词
手参/谱系地理学/遗传多样性/青藏高原Key words
Gymnadenia conopsea/phylogeography/genetic diversity/the Qinghai-Tibet Plateau引用本文复制引用
鲍武印,张阳,林鹏程,南蓬,黄艳燕,靳浩飞,钟扬..青藏高原植物手参的谱系地理学研究[J].生物技术通报,2016,32(12):96-102,7.基金项目
青海省自然科学基金项目 （）
