生物技术通报2016，Vol.32Issue(12)：103-107,5.DOI:10.13560/j.cnki.biotech.bull.1985.2016.12.017
版纳微型猪近交系 CATSPER3基因克隆、序列及发育 进化分析
Cloning,Sequence and Phylogenetic Analysis of CATSPER3 Gene in Banna Mini-pig Inbred Line
摘要
Abstract
Our purpose of this work is to obtain the partial coding region sequences of CATSPER3 gene from Banna Mini-pig Inbred Line(BMIL),and to analyze the amino acid sequence and homology comparison among different species by bioinformatics. We used boar testis material of BMIL to extract RNA,RT-PCR to amplify the coding sequence of CATSPER3 gene,and online analysis software to analyze the bioinformatics. Bioinformatics analysis showed that the protein molecular weight of deduced amino acid sequence was 19.397 3 kD and theoretical isoelectric point was 5.05. Regarding the composition of amino acid,there were 24 acidic amino acids(Asp and Glu);and there were 16 alkaline amino acid(Lys+Arg);among them the leucine(Leu)accounted for 13.3%,valine(Val)9.6%,threonine(Thr)9.0%, phenylalanine(Phe)8.4%,glutamate(Glu)7.2%,aspartic acid(Asp)7.2%,and their contents were high. Compared with ordinary pig,the nucleotide similarity was the highest,however,lower with that of goat. Molecular phylogenetic tree showed that inbred pig and non-inbred pig clustered in the same branch,i.e.,their genetic relationship was close,while far from goat and sheep.关键词
版纳微型猪近交系/CATSPER3基因/系统进化分析Key words
Banna Mini-pig Inbred Line/CATSPER3 gene/phylogenetic analysis引用本文复制引用
肖晶,曾养志,查星琴,成文敏,霍金龙,潘伟荣,王淑燕,王配,刘海京,张秀琼..版纳微型猪近交系 CATSPER3基因克隆、序列及发育 进化分析[J].生物技术通报,2016,32(12):103-107,5.基金项目
云南省教育厅科学研究基金项目 （）
