生物技术通报2016，Vol.32Issue(12)：166-171,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.12.026
一株产胞外多糖海洋弧菌的分离鉴定及其多糖抗肿瘤 活性初步研究
Isolation and Identification of Marine Vibrio Producing Extracellular Polysaccharide(EPS)and the Preliminary Study on Anti-tumor Activity of the EPS
摘要
Abstract
In order to investigate the bio-activities of the extracellular polysaccharide(EPS)from the marine bacteria in the Guangxi Beibu Gulf,the marine bacteria of EPS-producing were isolated from water/mud samples by LB-aniline blue medium. Phenol-sulfuric acid method was used for measuring the yield of the EPS. The marine bacterial strains with high-yield of ESP were further identified by the sequence analysis of 16S rDNA. The molecular weight and the preliminary structure of the EPS were tested by Gel permeation chromatography(GPC) and infrared spectrometry,respectively. Finally,the cytotoxicity of the EPS on Vero cells and the inhibitory effect of the EPS on Hela cells were tested by the MTT method. The results showed that totally 167 marine bacteria producing EPS were isolated from sea water/mud of mangrove,one of which(named BHc-09)yielded ESP as high as 0.306 mg/mL and was identified to be Vibrio by the analysis of its 16S rDNA sequence. The molecular weight of the EPS from BHc-09 was 184.5 kD,and there was uronic acid in the EPS structure. The EPS produced by BHc-09 presented no cytotoxicity on Vero cell,but significantly inhibitory effects on tumor cells. The results suggested that an EPS-producing marine Vibrio was successfully isolated and identified,and its extracellular polysaccharide has anti-tumor activity.关键词
海洋弧菌/胞外多糖/分离鉴定/抗肿瘤Key words
marine Vibrio/extracellular polysaccharide/isolation and identification/anti-tumor引用本文复制引用
龙寒,陈盛峰,陈佳,杨迪,李晓燕,黄玉油,何秀苗,禤金彩..一株产胞外多糖海洋弧菌的分离鉴定及其多糖抗肿瘤 活性初步研究[J].生物技术通报,2016,32(12):166-171,6.基金项目
国家自然科学基金项目（31560706），2014广西民族大学相思湖青年学者创新团队资助，广西林产化学与工程协同创新高水平 研究项目 （）
