生物技术通报2016，Vol.32Issue(12)：189-194,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.12.029
溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响
Effects of Lysophosphatidic Acid on the Migrations of Hepatoma Cells with Different Metastasis
摘要
Abstract
The aim of this study is to investigate the effect of lysophosphatidic acid(LPA)on cell migration of two kinds of hepatocellular carcinoma cells(MHCC97H and HepG2)with different metastasis,and to evaluate the underlying mechanism. Transwell assay was employed to investigate the impact of LPA on the migration of the hepatocellular carcinoma cells,and RT-PCR to detect the mRNA expression of LPA receptor(LPAR)in the hepatocellular carcinoma cells. Results showed that the LPA presented no obvious effect on the migration of HepG2 cells in low metastasis,but significantly promoted the migration of MHCC97H cells in high metastasis. LPA caused insignificant impact to the proliferation of both cells. RT-PCR demonstrated that expressions of LPAR at mRNA level in MHCC97H and HepG2 cells were different,LPAR1 expressed in MHCC97H cells,however,not in HepG2 cells. Importantly,the promotion effect of LPA on the migration of MHCC97H cells disappeared after Ki16425(a specific inhibitor of LPAR1/3)was used to block the role of LPAR1 in MHCC97H cells. This indicated that LPA promoted MHCC97H cell migration via interaction with LPAR1. The effects of LPA on the migration of hepatoma cells in different metastasis varied,which resulted from the different expressions of LPAR in these two cell types.关键词
溶血磷脂酸/肝癌细胞/溶血磷脂酸受体/细胞迁移Key words
lysophosphatidic acid/hepatoma cell/lysophosphatidic acid receptor/cell migration引用本文复制引用
李铎,李佳文,袁群琛,林川川,宋关斌..溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响[J].生物技术通报,2016,32(12):189-194,6.基金项目
国家自然科学基金项目（11272365），国家级大学生创新训练项目 （）
