山西农业大学学报（自然科学版）2016，Vol.36Issue(11)：760-767,785,9.
利用 ITS2序列识别盾蚧科昆虫
Identifying the armored scales (Hemiptera:Diaspididae)using ITS2 gene
摘要
Abstract
Objective]Armored scale is one of the family of coccoidea that most species are considered to be serious eco-nomic pests.However,identification of Diaspididae species is difficult due to the shortage of easily distinguishable morphological characters.As a useful tool for molecular species identification,DNA barcoding might offer potential u-sing for armored scale identification and taxonomy.[Methods]74 sequences belongs 1 6 species with distance-based method and tree-based method were employed to assess the effectiveness of ITS2 gene fragment.[Results]The result showed there was no barcode gap between intraspecies and interspecies distance.However,all phylogenetic trees ex-hibited similar topology and could distinguish all surveyed species in Diaspididae with high support values.BM and BCM all showed more than 90% identification success rate.[Conclusion]The study confirm ITS2 gene is effective for the identification of armored scale.关键词
盾蚧/ITS2/DNA 条形码/物种识别/分类Key words
Armored scale/Internal transcribed spacer 2/DNA barcoding/Species identification/Taxonomy引用本文复制引用
魏久锋,赵婉清,李荣荣,张虎芳..利用 ITS2序列识别盾蚧科昆虫[J].山西农业大学学报（自然科学版）,2016,36(11):760-767,785,9.基金项目
国家自然科学基金(31301899) （31301899）
山西农业大学科技创新基金(2015YJ03) （2015YJ03）
山西省科技攻关项目(20150311010-7) （20150311010-7）
