山西农业大学学报（自然科学版）2016，Vol.36Issue(11)：827-831,5.
马身猪和大白猪背最长肌 Rac 1基因发育性表达研究
Developmental expression of Rac 1 gene in longissimus dorsi in mashen and large white pigs
摘要
Abstract
Objective]The aim of this study was to investigate the developmental patterns of Rac 1 gene in longissimus dorsi and its influence on muscle growth and development in Mashen and Large White pigs.[Methods]The relative ex-pressions at the levels of mRNA and protein of Rac1 gene in longissimus dorsi at the ages of 1,30,60,90,120,1 50, and 180 days old in Mashen and Large White pigs were detected by quantitative real-time PCR and western blot.[Re-sults]The main results showed that the mRNA relative expression of Rac1 gene in longissimus dorsi at the ages of 1, 120,and 180 days old in Large White pig was greater than those at other ages (P <0.01),whose relative expression was low.In Mashen pig,the highest relative expression of Rac1 mRNA was at 60 day-old (P <0.01),then at 90 day-old.The expressions at other ages were kept low and stable levels and no significant difference was found among them. Concerning to the relative expression of Rac1 protein,the changing trend in Large White pig was decreasing and in-creasing from one day-old to 180 day-old,and the greatest peak was at one day-old,the lowest point was at 120 day-old.The developmental expression trend of Rac1 in Mashen pig was the opposite with that in Large White,and fol-lowed the trend of increasing and decreasing.The greatest relative expression was at 60 day-old,and significantly grea-ter than those at other ages (P <0.01).The relative expression amount was became low and stable since 60 day-old, and lower than those at one day-old and 30 day-old (P <0.05).[Conclusion]The relative expression of Rac1 gene at the levels of mRNA and protein was associated with age and genetic background of pigs.关键词
猪/Rac1 基因/发育性表达Key words
Pig/Rac1 gene/Developmental expression分类
农业科技引用本文复制引用
王泽艺,石建中,刘郑煜,高鹏飞,浦忠得,杨青春,郭晓红,李步高,曹果清..马身猪和大白猪背最长肌 Rac 1基因发育性表达研究[J].山西农业大学学报（自然科学版）,2016,36(11):827-831,5.基金项目
山西省科技攻关项目(20140311020-5) （20140311020-5）
山西省研究生教育创新项目(2015SY25) （2015SY25）
