山西农业大学学报(自然科学版)2016,Vol.36Issue(12):837-845,9.
谷子 WRKY 转录因子基因家族分析
Analysis ofWRKY transcription gene family in foxtail millet
摘要
Abstract
Objective]WRKY transcription factor is one of the biggest gene families that are important regulators of ma-jor plant processes,including growth,development,and responses to stress.However,there is limited information a-bout the numbers,structures and functions of those WRKY transcription factors in foxtail millet (Setaria italica (L.) P.Beauv.).[Methods]In this paper,the WRKY transcription factor gene family in foxtail millet were separated and i-dentified by bioinformatics,and the expression were analyzed by qRT-PCR.[Results]In our study,103 WRKY tran-scription factor-encoding genes were identified in the S .italica genome.The genes were named SiWRKY1 to Si-WRKY103 according to their order on the chromosomes.The expression among four different tissues was analyzed u-sing publicly available RNA sequencing data.The expression of SiWRKY genes in response to dehydration were ana-lyzed by qRT-PCR.[Conclusion]In conclusion,most SiWRKY genes (>80%)were up-regulated by drought stress,it may indicated that these genes play very important roles for drought-resistant in millet,and our conclusion will provide a set of candidates genes for the research of plants’response to drought stress.关键词
谷子/干旱胁迫/WRKY 转录因子/基因表达Key words
Foxtail millet/Drought stress/WRKY transcription factor/Gene expression分类
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邢国芳,张莉,冯万军,谢圣杰,贾亚涛,苑乂川,陈小雨..谷子 WRKY 转录因子基因家族分析[J].山西农业大学学报(自然科学版),2016,36(12):837-845,9.基金项目
国家自然科学基金(31501323) (31501323)
山西省高等学校创新基金(2015145) (2015145)