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基于 PEG 胁迫表达谱发掘调控谷子干旱胁迫的泛素连接酶E3基因

宋敏 马芳芳 侯思宇 张雁明 刘龙龙 耿潞阳 江璇宇 李红英 韩渊怀

山西农业大学学报(自然科学版)2016,Vol.36Issue(12):860-867,8.
山西农业大学学报(自然科学版)2016,Vol.36Issue(12):860-867,8.

基于 PEG 胁迫表达谱发掘调控谷子干旱胁迫的泛素连接酶E3基因

Ubiquitin ligase E3 genes mining based on expression profiles under PEG stress in foxtail millet

宋敏 1马芳芳 2侯思宇 1张雁明 1刘龙龙 2耿潞阳 1江璇宇 3李红英 1韩渊怀1

作者信息

  • 1. 山西农业大学 农学院,山西太谷 030801
  • 2. 特色杂粮种质资源发掘与育种改良山西省重点实验室,山西太谷 030801
  • 3. 特色杂粮种质资源发掘与育种改良山西省重点实验室,山西太谷 030801
  • 折叠

摘要

Abstract

Objective]Analysis of E3 genes in foxtail millet under drought stress.[Methods]In this paper,Gou Gou Mu Ji Ju and Jin Fen 1 6 based on expression profiles under PEG stress using NGS technology,1 1 differentially expressed ubiquitin ligase E3 genes were revealed.Through the prediction and analysis of physicochemical properties,functional domains,regulatory elements in promoters,expression difference,sequence alignment and phylogenetic analysis. [Results]We found that isoelectric point was between 4.7 8 ~8.8 8 ,functional domains mainly include ZF_UBR type, ZF_RING type,and U _ BOX type.The promoters sequence contained MBS,ABRE,etc.Phylogenetic showed that Si0 0 1 6 6 5 m and Si0 2 2 1 7 0 m were closely related to PUB2 2/2 3 ,Si0 2 9 0 9 0 m were closely ralated to PUB1 9. [Conclusion]Some ubiquitin ligase E3 genes,which may be induced by drought or ABA,were identified.This would provide a theoretical basis for further research of ubiquitin ligase E3 in response to drought in foxtail millet.

关键词

谷子/泛素连接酶 E3/干旱胁迫/表达谱

Key words

Foxtail millet/Ubiquitin ligase E3/Drought stress/Expression profile

分类

农业科技

引用本文复制引用

宋敏,马芳芳,侯思宇,张雁明,刘龙龙,耿潞阳,江璇宇,李红英,韩渊怀..基于 PEG 胁迫表达谱发掘调控谷子干旱胁迫的泛素连接酶E3基因[J].山西农业大学学报(自然科学版),2016,36(12):860-867,8.

基金项目

国家自然科学基金(31371693,31471556,31401396) (31371693,31471556,31401396)

山西农业大学学报(自然科学版)

OACSTPCD

1671-8151

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