山西农业大学学报(自然科学版)2016,Vol.36Issue(12):860-867,8.
基于 PEG 胁迫表达谱发掘调控谷子干旱胁迫的泛素连接酶E3基因
Ubiquitin ligase E3 genes mining based on expression profiles under PEG stress in foxtail millet
摘要
Abstract
Objective]Analysis of E3 genes in foxtail millet under drought stress.[Methods]In this paper,Gou Gou Mu Ji Ju and Jin Fen 1 6 based on expression profiles under PEG stress using NGS technology,1 1 differentially expressed ubiquitin ligase E3 genes were revealed.Through the prediction and analysis of physicochemical properties,functional domains,regulatory elements in promoters,expression difference,sequence alignment and phylogenetic analysis. [Results]We found that isoelectric point was between 4.7 8 ~8.8 8 ,functional domains mainly include ZF_UBR type, ZF_RING type,and U _ BOX type.The promoters sequence contained MBS,ABRE,etc.Phylogenetic showed that Si0 0 1 6 6 5 m and Si0 2 2 1 7 0 m were closely related to PUB2 2/2 3 ,Si0 2 9 0 9 0 m were closely ralated to PUB1 9. [Conclusion]Some ubiquitin ligase E3 genes,which may be induced by drought or ABA,were identified.This would provide a theoretical basis for further research of ubiquitin ligase E3 in response to drought in foxtail millet.关键词
谷子/泛素连接酶 E3/干旱胁迫/表达谱Key words
Foxtail millet/Ubiquitin ligase E3/Drought stress/Expression profile分类
农业科技引用本文复制引用
宋敏,马芳芳,侯思宇,张雁明,刘龙龙,耿潞阳,江璇宇,李红英,韩渊怀..基于 PEG 胁迫表达谱发掘调控谷子干旱胁迫的泛素连接酶E3基因[J].山西农业大学学报(自然科学版),2016,36(12):860-867,8.基金项目
国家自然科学基金(31371693,31471556,31401396) (31371693,31471556,31401396)