山西农业大学学报（自然科学版）2016，Vol.36Issue(12)：879-884,6.
基于 SRAP-PCR 技术开发荞麦种间特异标记及相关序列分析
Developed specific-markers and sequence analysis related with specific loci among 40 cultivars of Buck-wheat by SRAP-PCR molecular marker system
摘要
Abstract
[Objective]Most of these Buckwheat cultivars,its seeds were heterozygosity because of their selfing incom-patibility.Hence,it has important product value and reality significance that developed molecular marker to identify different varieties of Buckwheat cultivars and seeds purity.[Methods]According to these DNA polymorphism loci a-mong 40 Buckwheat cultivars through SRAP-PCR system,we recycled and resequenced these different DNA loci,and analyzed their sequence character by bioinformation method.[Results]8 DNA polymorphism loci were cloned.Only 2 loci could find forward and reverse primer sequences and single primer in any other loci.So the 2 loci sequence were named 8801-1 and Mhua 5. The sequence length of 8801-1 was 705bp,and included 3 restriction Enzyme cutting site. 1 ORF domain was predicted in this sequence.Through BLASTP analysis,it had not find any homologous protein with this sequence.The sequence length of Mhua5 was 687bp,and included 3 restriction Enzyme cutting site.2 ORF do-main was predicted in this sequence.Through BLASTP analysis,there was 71% homology between the ORF2 in buck-wheat and retrotransposon pol protein in Arabidopsis thaliana .[Conclusion]The results suggested that this sequence could be included a retrotransposon element of gene.In summary,we have got 2 special DNA loci related with 2 Buck-wheat cultivars.One locus included retrotransposon element,anther locus was redundancy genome sequence.We hope to develop the 2 loci for STS markers,and apply them to analyze genetic diversity,and identify seeds purity,and con-struct genetic mapping and marker-assisted selection (MAS)of Buckwheat cultivars.关键词
甜荞/DNA 多态性位点/SRAP-PCR 技术/分子标记Key words
Buckwheat/Molecular marker assisted selection/SRAP-PCR/Sequence analysis分类
农业科技引用本文复制引用
王丽,孙朝霞,刘荣华,侯思宇,李红英,韩渊怀,黄可胜..基于 SRAP-PCR 技术开发荞麦种间特异标记及相关序列分析[J].山西农业大学学报（自然科学版）,2016,36(12):879-884,6.基金项目
国家自然科学基金(NSFC:31301385) （NSFC:31301385）
山西省科技攻关项目(20150311007-1) （20150311007-1）
