中国现代医学杂志2016，Vol.26Issue(22)：7-12,6.DOI:10.3969/j.issn.1005-8982.2016.22.002
神经降压素受体1在大鼠肺缺血再灌注损伤中的表达及作用
Effect of NTR1 expression in rats with lung ischemia-reperfusion injury and mechanism
摘要
Abstract
Objective To investigate neurotensin receptor 1 (NTR1) expression and the effect and mechanism of NTR1 expression in rats with lung ischemia-reperfusion injury (LIRI). Methods Forty SD rats were randomly grouped as Sham group, LIRI group, LIRI+saline control group, LIRI+DMSO control group, LIRI+TLR4-activated group, LIRI+TAK-242 group (TLR4-inhibited group), LIRI+NTR1-activated group and LIRI+ NTR1-inhibited group. The NTR1 expression and the role of NTR1 in the LIRI rats were detected with RT-PCR and Western blot and statistically analyzed. Results NTR1 mRNA and protein expressions in the LIRI group were significantly increased comparing with the sham group ( <0.05). NT further increased the levels of inflammatory cytokines, apoptosis and lung pathological injury in the LIRI group, while SR48692 significantly reduced the above changes ( < 0.05). Compared with the LIRI group, NTR1 expression was further enhanced in the LIRI+LPS group and was significantly inhibited in the LIRI+TAK-242 group ( <0.05). HIF-1αmRNA and protein expressions were significantly inhibitedby SR48692 and was significantly enhanced by NT compared with the LIRI group( <0.05). Conclusions There is a close relationship between NTR1 and LIRI. NT-NTR1 is involved in the pathogenesis of LIRI. There may be TLR4-NTR1-HIF-1α signal pathway in LIRI and the theoretical basis is provided to find new effective therapeutic targets in LIRI.关键词
大鼠/肺/缺血再灌注损伤/NTR1/TLR4Key words
rats/lung/ischemia-reperfusion injury/NTR1/TLR4分类
医药卫生引用本文复制引用
周志毅,朱幸沨,孙洁,陈静瑜,杨国仪..神经降压素受体1在大鼠肺缺血再灌注损伤中的表达及作用[J].中国现代医学杂志,2016,26(22):7-12,6.基金项目
无锡市医院管理中心重大扶持项目（NoYGZF1110）；江苏省人体器官移植重点实验室开放课题 （）
