中国现代医学杂志2016，Vol.26Issue(22)：18-22,5.DOI:10.3969/j.issn.1005-8982.2016.22.004
白藜芦醇对人前列腺癌细胞株PC-3增殖及TGF-β1/Smad2信号通路影响研究
Effect of resveratrol on proliferation and TGF-β1 signaling pathway on human prostate cancer cell line PC-3
王享利1
作者信息
- 1. 中南大学湘雅医院，湖南 长沙 410008
- 折叠
摘要
Abstract
Objective To observe the effect of resveratrol on proliferation and TGF-β1 signaling pathway of human prostate cancer cell line PC-3. Methods Human prostate cancer cell line PC-3 was cultured and treated with resveratrol at different concentrations and different time points. The effect of resveratrol on PC-3 cell proliferation was studied by means of MTT. The cell cycle was detected with flow cytometry. RT-PCR and Western blot were used to detect the expressions of TGF-β1 and Smad2 at mRNA and protein levels. Results The viability of Huh-7 cells treated with resveratrol was obviously decreased. Analysis of the cell cycle revealed that resveratrol induced a significant decrease in cells in the S phase and increase in cells in the G1 phase. Furthermore, the expressions of TGF-β1 and its downstream factor-Smad2 were inhibited at mRNA and protein levels by resveratrol in a concentration- and time-dependent manner. Conclusions Resveratrol perhaps plays inhibitory effect on PC-3 cells through inhibiting the activity of TGF-β1 signaling pathway.关键词
白藜芦醇/转化生长因子β1/前列腺癌Key words
resveratrol/TGF-β1 signaling pathway/prostate cancer分类
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王享利..白藜芦醇对人前列腺癌细胞株PC-3增殖及TGF-β1/Smad2信号通路影响研究[J].中国现代医学杂志,2016,26(22):18-22,5.
