中国现代医学杂志2016，Vol.26Issue(22)：23-27,5.DOI:10.3969/j.issn.1005-8982.2016.22.005
MT-3通过激活NF-κB信号通路抑制食管癌病理进展
MT-3 aggravates pathological process of esophageal cancer through activation of NF-κB signaling passway
王海军 1陆欣 2张彦芬 1刘聚良 1韩永强1
作者信息
- 1. 河北医科大学附属邢台人民医院 胸外科，河北 邢台 054001
- 2. 河北医科大学附属邢台人民医院 血液科，河北 邢台 054001
- 折叠
摘要
Abstract
Objective To research the development mechanism of esophageal cancer. Methods Ninety-two cases of esophageal cancer patients treated in Xingtai Pepole's Hospital of Hebei Medical University from July 2009 to February 2011 were selected for the study. MT-3 expression was detected in esophageal cancer tissues and the peri-cancerous tissues. MT-3 overexpression and low-expression cell lines were constructed. The activation state of NF-κB signaling pathway was checked at different levels of MT-3 expression. Then the expressions of P65 and pP65 were tested by Western blot. Immunofluorescence was used to further test whether pP65 was expressed in the nuclei when MT-3 was over-expressed. Results The expression of MT-3 in the tumor tissues was higher than that in the adjacent tissues ( = 0.001). A high MT-3 expression esophageal cancer cell line and a low MT-3 expression cell line were successfully constructed. pP65 protein expression was enhanced when MT-3 was over-expressed, but decreased at low MT-3 expression; in contrast, P65 showed the opposite results with significant differences, whichsuggested NF-κB signal was activated when MT-3 was over expressed. Immunofluorescence revealed that in MT-3 overexpression cells, more pP65 was expressed in the nuclei, which further supported that MT-3 activated NF-κB signaling pathway. Conclusions MT-3 suppresses the pathological process of esophageal cancer through activation of NF-κB signals.关键词
MT-3/NF-κB/信号通路/食管癌Key words
MT-3/NF-κB/esophageal cancer分类
医药卫生引用本文复制引用
王海军,陆欣,张彦芬,刘聚良,韩永强..MT-3通过激活NF-κB信号通路抑制食管癌病理进展[J].中国现代医学杂志,2016,26(22):23-27,5.
