中国药房2016，Vol.27Issue(34)：4780-4783,4.DOI:10.6039/j.issn.1001-0408.2016.34.09
鹿茸多肽诱导心肌干细胞向心肌细胞分化的机制研究
Study on the Mechanism of Differentiation from Cardiac Stem Cells into Cardiac Cells Induced by Cornu Corvi Polypeptides
摘要
Abstract
OBJECTIVE:To explore the mechanism of differentiation from cardiac stem cells (CSCs) into cardiac cells in-duced by Cornu Corvi polypeptides. METHODS:CSCs were isolated from SD rats,and cultured to P3 generation after identifica-tion and then divided into blank control group,5-azacitidine induction group (3 μmol/L),Cornu Corvi polypeptides induction group(800 μg/ml)and combination induction group. Except blank control group was cultured with culture solution containing 3%FBS,other groups were cultured with culture solution containing relevant derivant for 14 d. The expression of cTnT was deter-mined by immunohistochemical method;the expression of cTnT,ATF-2 and MEF-2C were determined by immunoblot assay;mRNA expression of cTnT,Nkx2.5 and GATA4 were detected by RT-PCR. RESULTS:CSCs were prepared successfully with puri-ty>97%. Compared with blank control group(cTnT being negative),cTnT of induction groups were positive,and the protein ex-pression of cTnT,ATF-2 and MEF-2C and mRNA expression of cTnT,Nkx2.5 and GATA4 were all increased (P<0.05). Com-pared with 5-azacitidine induction group,the protein expression of cTnT and MEF-2C and mRNA expression of cTnT and GATA4 were decreased in Cornu Corvi polypeptide groups(P<0.05);the protein expression of cTnT,ATF-2 and MEF-2C and mRNA ex-pression of cTnT and GATA4 were all increased in combination induction group (P<0.05). CONCLUSIONS:Cornu Corvi poly-peptides can induce differentiation of CSCs into cardiac cells by promoting the expression of Nkx2.5,GATA4,ATF-2,MEF-2C and other transcription factors.关键词
鹿茸多肽/心肌干细胞/心肌细胞/分化Key words
Cornu Corvi polypeptides/Cardiac stem cells/Cardiac cells/Differentiation分类
医药卫生引用本文复制引用
王艳玲,黄晓巍,李哲,何璐,徐岩,曲晓波..鹿茸多肽诱导心肌干细胞向心肌细胞分化的机制研究[J].中国药房,2016,27(34):4780-4783,4.基金项目
吉林省中医药科技项目（No.2014ZD6）；吉林省卫生计生科研计划项目 （）
