中国药房2016，Vol.27Issue(34)：4804-4806,3.DOI:10.6039/j.issn.1001-0408.2016.34.16
封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究
Study on in vitro Antioxidant,Anticoagulant and α-glucosidase Inhibitory Activities of Different Extracts of Lonicerae japonicae from Fengqiu
朱小峰 1朱晓娣 2王金梅1
作者信息
- 1. 河南大学中药研究所，河南开封475004
- 2. 赛诺菲(中国)投资有限公司上海分公司，上海200040
- 折叠
摘要
Abstract
OBJECTIVE:To investigate in vitro antioxidant,anticoagulant andα-glucosidase inhibitory activities of different ex-tracts of Lonicerae japonicae from Fengqiu. METHODS:The total extract was obtained from L. japonicae by extraction with 30%ethanol,and dispersed in water,from which the extracts of corresponding parts were obtained after extraction successively with tri-chloromethane and ethyl acetate. The in vitro antioxidant activity of different extracts from L. japonicae [reflected by IC50,Trolox equivalent value] was investigated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,2,2′-azinobis-(3-ethylbenzthiazoline-6-sulpho-nate)(ABTS)radical scavenging test and Fe3+ reduction and antioxidant capacity test. The α-glucosidase inhibitory activity and co-agulant activity of 4 extracts were also studied in vitro. RESULTS:The ethyl acetate extract of L. japonicae had the highest antioxi-dant activity among 4 extracts,IC50 of ABTS and DPPH were 9.22,25.23 µg/ml and Trolox equivalent value was (2 480.80 ± 5.51)μmol/g. Procoagulant activity of water extract and anticoagulation activity of n-butyl alcohol extract were the highest;the plasma recalcification time were(210.28±2.19)s and(149.20±1.25)s. Both total extract and ethyl acetate part inhibited the activ-ity of α-glucosidase,and IC50 of re-screening were 378.61,451.45 μg/ml. CONCLUSIONS:L. japonicae from Fengqiu have good antioxidant,anticoagulant andα-glucosidase inhibitory activities in vitro,and could be further developed and studied.关键词
金银花/封丘/抗氧化/凝血/α-葡萄糖苷酶/体外Key words
Lonicerae japonicae/Fengqiu/Antioxidant/Coagulant/α-glucosidase/in vitro分类
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朱小峰,朱晓娣,王金梅..封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究[J].中国药房,2016,27(34):4804-4806,3.
