中国药房2016，Vol.27Issue(34)：4845-4847,3.DOI:10.6039/j.issn.1001-0408.2016.34.29
表柔比星聚乳酸-羟基乙酸共聚物微球的制备及抗肿瘤活性研究
Study on Preparation and Antitumor Activity of Epirubicin-loaded PLGA Microspheres
刘炜 1王晓彤 1刘菊1
作者信息
- 1. 首都医科大学附属北京世纪坛医院药剂科，北京 100038
- 折叠
摘要
Abstract
OBJECTIVE:To prepare epirubicin-loaded PLGA microspheres and study its in vitro antitumor activity. METH-ODS:Using PLGA as carrier,epirubicin-loaded PLGA microspheres were prepared by using emulsification-solvent evaporation method. The morphology of microspheres was observed by TEM;the distribution of particle size was determined by laser granulari-ty distribution measuring instrument;the content of main component was determined by UV spectrophotometry. The drug-loading amount and entrapment efficiency were calculated,and accumulative release rate(Q)was investigated by in vitro release test with-in 10 d. The proliferation inhibitory rates of prepared microspheres and epirubicin to human breast cancer MCF-7 cells were detect-ed by MTT assay. RESULTS:The particle size,drug-loading amount and encapsulation efficiency of epirubicin-loaded PLGA mi-crospheres were(175.2±16.8)μm,(8.6±1.3)% and(46.7±8.6)%(n=7);Q4 h,Q24 h and Q10 d were 27.8%,41.7% and 92.3%, respectively. Compared with epirubicin,epirubicin-loaded PLGA microspheres could prolong inhibitory rate of epirubicin to MCF-7 cells for 96 h (48 h inhibitory rate of epirubicin was 96.7%,96 h proliferation inhibitory rate of Epirubicin-loaded PLGA micro-spheres was 99.3%). CONCLUSIONS:Epirubicin-loaded PLGA microspheres are prepared successfully,and show good in vitro sustained-release effect and antitumor activity.关键词
表柔比星/聚乳酸-羟基乙酸共聚物/微球/抗肿瘤活性Key words
Epirubicin/PLGA/Microspheres/Antitumor activity分类
医药卫生引用本文复制引用
刘炜,王晓彤,刘菊..表柔比星聚乳酸-羟基乙酸共聚物微球的制备及抗肿瘤活性研究[J].中国药房,2016,27(34):4845-4847,3.
