摘要
Abstract
OBJECTIVE:To separate and purify oligosaccharide and polysaccharide from the flowers of Magnolia officinalis, and to investigate their in vitro antioxidant capacity. METHODS:Using 80% ethanol as extraction solvent,the flowers of M. offici-nalis were extracted by reflux extraction and purified by column chromatogram to obtain oligosaccharide. The dregs of oligosaccha-ride extraction was used as raw material,and were extracted by hot water reflux extraction and purified by segmentation purifica-tion to obtain polysaccharide. Using 2,6-di-tert-butyl-4-methylphenol(BHT)as positive control,in vitro antioxidant capacity of dif-ferent concentrations of oligosaccharide and polysaccharide from the flowers of M. officinalis (0.1,0.2,0.4,0.6,0.8,1.0,1.2 mg/ml)were investigated through clearance rate test,reducing capacity test,total antioxidant activity test of 1,1-diphenyl-2-trinitro-phenylhydrazine (DPPH) free radical. RESULTS:DPPH free radical clearance,reducing capacity and total antioxidant activity were enhanced as the increase of oligosaccharide and polysaccharide concentration,when the concentration of oligosaccharide and polysaccharide ranged 0.1-1.0 mg/ml. In 3 tests,antioxidant activity index of oligosaccharide were similar to those of BHT (P>0.05) when the concentrations were 0.8-1.0,0.2-1.0,0.1-1.0 mg/ml. In 3 tests,most of antioxidant activity index of oligosaccha-ride were slightly higher than those of polysaccharide,without statistical significance (P>0.05). CONCLUSIONS:The oligosac-charide and polysaccharide from the flowers of M. officinalis show good in vitro antioxidant capacity,and in vitro antioxidant capac-ity of oligosaccharide is better than that of polysaccharide.关键词
厚朴花/低聚糖/多糖/分离/纯化/抗氧化活性Key words
Flowers of Magnolia officinalis/Oligosaccharide/Polysaccharide/Separation/Purification/Antioxidant activity分类
医药卫生
