中国医院用药评价与分析2016，Vol.16Issue(11)：1466-1468,1469,4.DOI:10.14009/j.issn.1672-2124.2016.11.007
两种定值方法测定阿司匹林丁香酚酯对照品的含量
Content Determination of Aspirin Eugenol Ester Reference Substance by Two Measuring Methods
摘要
Abstract
OBJECTIVE:To determine the content of aspirin eugenol ester (AEE) reference substance by by the mass balance method and the nuclear magnetic resonance ( NMR) method.METHODS:Chromatogram purity was determined by HPLC, the moisture and volatile substance were determined by weightlessness method and inorganic impurities was determined by the residue on ignition method .The content of AEE reference substance was calculated by the mass balance method.NMR quantification method for AEE was established with determine temperature of 25℃, scan times of 16 and the relaxation delay time of 15 s respectively .RESULTS:The results of content determination were 99.82%by the mass balance method and 99.55%by the NMR method .Fitting line between peak area ratio and weight ratio of sample AEE to inner standard(1,4-dinitrobenzene) ranged from 3 mg to 21 mg with correlative coefficient of 0.999.Method validation of the NMR about the precision RSD was 0.04%(n=5)and the repeatability RSD was 0.19%(n=5)and the stability of RSD was 0.57%.CONCLUSIONS:Two methods can verify each other and the determination results are basically identical .关键词
质量平衡法/核磁共振法/对照品/阿司匹林丁香酚酯/方法验证Key words
Mass balance method/NMR method/Reference substance/Aspirin eugenol ester/Aspirin分类
医药卫生引用本文复制引用
杨青青,刘希望,杨亚军,李剑勇,查飞..两种定值方法测定阿司匹林丁香酚酯对照品的含量[J].中国医院用药评价与分析,2016,16(11):1466-1468,1469,4.基金项目
中国农业科学院科技创新工程 （）
