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藤茶泡水前后的抗菌活性和抗氧化活性比较

刘熠杭 马玉杰 商艳君 尹健铎

安徽农业科学2016,Vol.44Issue(33):113-115,3.
安徽农业科学2016,Vol.44Issue(33):113-115,3.

藤茶泡水前后的抗菌活性和抗氧化活性比较

Antimicrobial Activity and Antioxidant Activity Comparison of Ampelopsis grossedentata before and after Soaking in Water

刘熠杭 1马玉杰 2商艳君 2尹健铎2

作者信息

  • 1. 河北省石家庄市第一中学,河北石家庄050010
  • 2. 河北经贸大学生物科学与工程学院,河北石家庄050061
  • 折叠

摘要

Abstract

Objective] To compare antibacterial activity and antioxidant activity of ethanol extracts from Ampelopsis grossedentata before and after soaking in water.[Method]The folin-ciocalteu method was used to make the total phenolic content, antibacterial and antifungal activities were done by the inhibition zone method and the fungus cake method respectively, and antioxidant activity was done by DPPH method.[Re-sult] The total phenolic contents of ethanol extract of Ampelopsis grossedentata ( EEAG ) before and after soaking in water were 168 and 56 kg/g.EEAG before soaking in water showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Botrytis cinerea, Colletotrichum gloeosporioides and Coniothyrium diplodiella.EEAG after soaking in water exhibited no antimicrobial activity against Bacillus subtilis and lower antimicrobial activities against the other five microbes.The IC50 values of DPPH radical scavenging ability of EEAG before and after soaking in waer were 5.09 and 36.01 μg/mL respectively.[Conclution]There are certain antimicrobial and antioxidant activi-ties of the residue of Ampelopsis grossedentata after soaking in water, so it should still be exploited.

关键词

藤茶/抗细菌活性/抗真菌活性/抗氧化活性/总酚含量

Key words

Ampelopsis grossedentata/Antibacterial activity/Antifungal activity/Antioxidant activity/Total phenolic content

分类

农业科技

引用本文复制引用

刘熠杭,马玉杰,商艳君,尹健铎..藤茶泡水前后的抗菌活性和抗氧化活性比较[J].安徽农业科学,2016,44(33):113-115,3.

基金项目

2016年博士科研启动经费(0112160225);2014省教育厅项目(2311002027)。 ()

安徽农业科学

0517-6611

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