安徽农业科学2016,Vol.44Issue(33):113-115,3.
藤茶泡水前后的抗菌活性和抗氧化活性比较
Antimicrobial Activity and Antioxidant Activity Comparison of Ampelopsis grossedentata before and after Soaking in Water
摘要
Abstract
Objective] To compare antibacterial activity and antioxidant activity of ethanol extracts from Ampelopsis grossedentata before and after soaking in water.[Method]The folin-ciocalteu method was used to make the total phenolic content, antibacterial and antifungal activities were done by the inhibition zone method and the fungus cake method respectively, and antioxidant activity was done by DPPH method.[Re-sult] The total phenolic contents of ethanol extract of Ampelopsis grossedentata ( EEAG ) before and after soaking in water were 168 and 56 kg/g.EEAG before soaking in water showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Botrytis cinerea, Colletotrichum gloeosporioides and Coniothyrium diplodiella.EEAG after soaking in water exhibited no antimicrobial activity against Bacillus subtilis and lower antimicrobial activities against the other five microbes.The IC50 values of DPPH radical scavenging ability of EEAG before and after soaking in waer were 5.09 and 36.01 μg/mL respectively.[Conclution]There are certain antimicrobial and antioxidant activi-ties of the residue of Ampelopsis grossedentata after soaking in water, so it should still be exploited.关键词
藤茶/抗细菌活性/抗真菌活性/抗氧化活性/总酚含量Key words
Ampelopsis grossedentata/Antibacterial activity/Antifungal activity/Antioxidant activity/Total phenolic content分类
农业科技引用本文复制引用
刘熠杭,马玉杰,商艳君,尹健铎..藤茶泡水前后的抗菌活性和抗氧化活性比较[J].安徽农业科学,2016,44(33):113-115,3.基金项目
2016年博士科研启动经费(0112160225);2014省教育厅项目(2311002027)。 ()