安徽农业科学2016，Vol.44Issue(33)：140-142,3.
YDL080 c基因缺失的低异戊醇酿酒酵母菌构建
Construction of Saccharomyces cerevisiae with Low Isopentanol Production by Deleting Gene YDL080c
杨青 1李洲 2周世水1
作者信息
- 1. 华南理工大学生物科学与工程学院，广东广州510006
- 2. 汕尾市青梅产业研究院，广东陆丰516500
- 折叠
摘要
Abstract
Objective]The Saccharomyces cerevisiae with producing low isopentanol was constructed by knocking out geneYDL080c.[Method] The Saccharomyces cerevisiae with producing low isopentanol was constructed byusing Cre-loxP recombination system and yeast electrical trans-formation method to knock out the gene YDL080c encoding pyruvate decarboxylase,in order to cut off the produce of isopentanol in metabolism of Saccharomyces cerevisiae.[ Result] The engineering strain Y1 without YDL080c was successfully constructed.The relative isopentanol content was 0.81 g/L in wine brewed by Y1, which was 28.3% lower than that of the original strain.[Conclusion]Engineering Saccharomyces cerevi-siae without gene YDL080c can effectively reduce the production of isopentanol in brewed wine.关键词
基因敲除/异戊醇/酿酒酵母Key words
Gene knock-out/Isopentanol/Saccharomyces cerevisiae分类
农业科技引用本文复制引用
杨青,李洲,周世水..YDL080 c基因缺失的低异戊醇酿酒酵母菌构建[J].安徽农业科学,2016,44(33):140-142,3.
